Targeted delivery and apoptosis induction activity of peptide-transferrin targeted mesoporous silica encapsulated resveratrol in MCF-7 cells

作者全名："Li, Dongning; Song, Chengzhu; Zhang, Jie; Zhao, Xiaoyan"

作者地址："[Li, Dongning; Song, Chengzhu; Zhao, Xiaoyan] Southwest Univ, Inst Pharmaceut Sci, Chongqing 400716, Peoples R China; [Zhang, Jie] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Zhao, XY (通讯作者)，Southwest Univ, Coll Pharmaceut Sci, Chongqing 400716, Peoples R China."

来源：JOURNAL OF PHARMACY AND PHARMACOLOGY

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000861515700001

JCR分区：Q2

影响因子：3.3

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：breast cancer; resveratrol; mesoporous silica; transferrin

摘要："Objectives Resveratrol (Res) was a naturally occurring polyphenol compound. It has various beneficial effects, including anti-inflammatory, anti-oxidant and anti-cancer effects. However, the anti-cancer activity was hindered by its low targeting and drug release performance. Thus, we synthesized transferrin-cathepsin B cleavable peptide modified mesoporous silica nanoparticle encapsulated Res (Tf-Res-MSN). Methods Res was encapsulated in mesoporous silica nanoparticles (MSN), which was a kind of drug carrier complex. Tf was modified to recognize the cancer cells. Cathepsin B cleavable peptide (Pep) was used to combine Res-MSN complex and Tf to construct the final product. Pep was used as linker and trigger for Res release. Key findings The smart nanocarriers were increased the drug release performance of Res in human breast cancer (MCF-7) cells. The physicochemical properties of Tf-Res-MSN were assessed by zeta potential, UV-Prove, diffraction scanning calorimetry (DSC), nitrogen physisorption analysis and transmission electron microscope (TEM). MTT assay, AO and Annexin V-FITC/PI staining were performed to explore the anti-tumour activity of Tf-Res-MSN. The results showed that Tf-Res-MSN significantly decreased cell viability and increased cell apoptosis. The inhibition rate and apoptotic rate of Tf-Res-MSN in MCF-7 cells were 95.75% and 80.8%, respectively. Conclusion Our study demonstrated that Tf-Res-MSN was a valuable technique with potential value in breast cancer applications."

基金机构："Chongqing Research Program of Basic Research and Frontier Technology, China [cstc2021jcyjmsxmX0106]; Chinese Medicine Rehabilitation the Key Discipline Constructed by Chongqing Health Bureau [2021-4322190044]"

基金资助正文："The present study was supported by the Chongqing Research Program of Basic Research and Frontier Technology, China (grant no. cstc2021jcyjmsxmX0106) and Chinese Medicine Rehabilitation the Key Discipline Constructed by Chongqing Health Bureau (grant no. 2021-4322190044)."