A simple and smart AND-gate DNA nanoprobe for correlated enzymes tracking and cell-selective imaging

作者全名："Zeng, Zijie; Guo, Bianqin; Li, Xingrong; Fu, Xuhuai; Chen, Yirong; Wu, Haiping; Zhang, Lu; Chen, Junman; Ding, Shijia"

作者地址："[Zeng, Zijie; Li, Xingrong; Chen, Yirong; Wu, Haiping; Zhang, Lu; Chen, Junman; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diag, Minist Educ, Chongqing 400016, Peoples R China; [Guo, Bianqin; Fu, Xuhuai] Chongqing Univ, Key Lab Translat Res Canc Metastasis & Individuali, Canc Hosp, Chongqing 400030, Peoples R China"

通信作者："Ding, SJ (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diag, Minist Educ, Chongqing 400016, Peoples R China."

来源：BIOSENSORS & BIOELECTRONICS

ESI学科分类：CHEMISTRY

WOS号：WOS:000863428200006

JCR分区：Q1

影响因子：12.6

年份：2022

卷号：217

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Telomerase; APE1; Simultaneous detection; Cell imaging; AND-Gate logic strategy

摘要："Accurate cancer diagnosis and effective drug therapy entail sensitive and dynamic monitoring of intracellular key enzymes, since their expression level is closely related to disease progression. Simultaneous monitoring of correlated enzymes is promising to help unveiling mystery of cytobiological events during tumor progression and drug response, while is challenged by lacking of a robust and simple simultaneous detection strategy. In order to construct a simple and smart strategy which is complex design-avoided and doesn't need other auxiliary enzyme, here we develop an AND-gate strategy for simultaneously monitoring correlated enzymes which both are upregulated in cancer cells (telomerase and apurinic/apyrimidinic endonuclease 1). An innovative AND-gate DNA nanoprobe has been designed to avoid mutual interference and background noise, guaranteeing an enhanced fluorescent signal output upon catalyzation of dual enzymes. This AND-gate strategy achieves sensitive detection of two enzymes in an individual manner in test tube, through which the diagnostic potential of bladder cancer has been validated by telomerase detection in clinical urine sample. The AND-gate strategy enables specific intracellular imaging of dual enzymes in different cancer cell lines. Importantly, in contrast to traditional single-targeting strategies, AND-gate imaging of dual enzymes significantly improves cancer cell selectivity. Moreover, this strategy dynamically monitors enzymatic activity changes during chemoresistance induced by chemotherapeutic treatment. This simple and smart strategy has foreseeable prospect in the fields of disease diagnosis, drug prognosis evaluation, and precise fluorescence-guided surgery."

基金机构：NSFC; China Postdoctoral Science Foundation; Special Funding for Postdoctoral Research Projects (Chongqing); [2021M700630]

基金资助正文：This work is supported by NSFC 81873980; Special Funding for Postdoctoral Research Projects (Chongqing) ; China Postdoctoral Science Foundation (2021M700630) .