Exo-III Enzyme and DNAzyme-Assisted Dual Signal Recycles for Sensitive Analysis of Exosomes by Using Personal Glucose Meter

作者全名："Hu, Wenyi; Su, Hailong; Zeng, Xiaojuan; Duan, Xinglian; Li, Yangqiu; Li, Li"

作者地址："[Hu, Wenyi] 958 Hosp PLA Army, Dept Cardiol, Chongqing 400020, Peoples R China; [Su, Hailong; Zeng, Xiaojuan; Duan, Xinglian; Li, Yangqiu; Li, Li] Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 3, Chongqing 401120, Peoples R China"

通信作者："Li, L (通讯作者)，Chongqing Med Univ, Dept Cardiol, Affiliated Hosp 3, Chongqing 401120, Peoples R China."

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000865899700002

JCR分区：Q3

影响因子：3

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Personal glucose meters; Exosomes; Exo-III enzyme; Sucrase

摘要："Exosome plays a crucial role in regulating intercellular communication during atherosclerosis development. However, sensitive and portable exosome detection remains a huge challenge. Herein, a personal glucose meter (PGM)-based exosomes detection approach has been proposed that allows detection of exosomes with a high sensitivity and reproducibility. In this method, a catch probe, which is composed of CD63 aptamer and blocker sequence, is utilized for the specific identification of exosomes. The blocker sequence binds with H probe to initiate the Exo-III-assisted signal recycles to generate numerous DNAzyme sequences. Under the assistance of the substrate, DNAzyme forms its active secondary structure to generate gap site in substrate, releasing a linker to conjugate sucrase to streptavidin magnetic beads (SMBs). After removing unbound sucrase, the SMB-linker-sucrase complex is used to catalyze sucrose to glucose, which can be read by PGMs. Based on this, the method exhibits a wide detection range and a low limit of detection, holding a promising prospect for the analysis of exosomes and screening atherosclerosis."

基金机构：Third Affiliated Hospital of Chongqing Medical University

基金资助正文：The authors thank the financial and technical support from The Third Affiliated Hospital of Chongqing Medical University.