TCDD induces cleft palate through exosomes derived from mesenchymal cells

作者全名："Chen, Qiang; Xie, Yue; Dong, Xiaobo; Zhang, Xiao; Zhang, Yunxuan; Yuan, Xingang; Ding, Xionghui; Qiu, Lin"

作者地址："[Chen, Qiang; Xie, Yue; Dong, Xiaobo; Zhang, Xiao; Zhang, Yunxuan; Yuan, Xingang; Ding, Xionghui; Qiu, Lin] Chongqing Med Univ, Key Lab Child Dev & Disorders, Chongqing Key Lab & Pediat,Natl Clin Res Ctr Chil, Minist Educ,Dept Burn & Plast Surg,Childrens Hosp, Chongqing 400000, Peoples R China; [Chen, Qiang] Chongqing Univ, Three Gorges Hosp, Dept Pediat Surg, Chongqing 400000, Peoples R China"

通信作者："Qiu, L (通讯作者)，Chongqing Med Univ, Dept Burn & Plast Surg, Childrens Hosp, 136 Zhongshan Second Rd, Chongqing 400000, Peoples R China."

来源：TOXICOLOGY RESEARCH

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000866463200001

JCR分区：Q4

影响因子：2.1

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：2; 3; 7; 8-tetrachlorodibenzo-p-dioxin; exosomes; proliferation; migration; apoptosis; epithelial-mesenchymal transition

摘要："TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin) is a ubiquitous environmental toxicant and a notable teratogenic agent for cleft palate (CP), a common congenital structural malformation that can result from abnormalities during palatal shelf connection and/or fusion. The development of the palate requires precise coordination between mesenchymal and epithelial cells. Exosomes are vesicles secreted by cells and participate in organ development by transferring various bioactive molecules between cells and regulating cell proliferation, migration, apoptosis, and epithelial-mesenchymal transition (EMT); these vesicles represent a new method of intercellular communication. To explore how TCDD could influence palatal cell behaviors and communication, we treated mesenchymal cells with TCDD, collected the exosomes secreted by the cells, assessed the 2 types of palatal cells, and then observed the effects of TCDD-induced exosomes. We found that the effects of TCDD-induced exosomes were equal to those of TCDD. Thus, TCDD might change the genetic materials of palatal cells and exosomes to cause dysregulated gene expression from parental cells, affect cellular information communicators, and induce abnormal cellular behaviors that could lead to CP."

基金机构：Chongqing Returned Overseas Students Innovation Plan Fund [cx2019134]; Chongqing Natural Science Foundation [cstc2020jcyj-msxmX0228]

基金资助正文：The Chongqing Returned Overseas Students Innovation Plan Fund (cx2019134) and the Chongqing Natural Science Foundation (cstc2020jcyj-msxmX0228).