Protective effects of glutamine on lipopolysaccharide/D-galactosamine-induced fulminant hepatitis in mice

作者全名："Yang, Mengxin; Zhang, Xinyue; Zhao, Shuang; Shao, Ruyue; Fan, Kerui; Hu, Kai; Zhang, Li; Yang, Yongqiang"

作者地址："[Yang, Mengxin; Zhang, Xinyue; Zhao, Shuang; Fan, Kerui; Hu, Kai; Zhang, Li; Yang, Yongqiang] Chongqing Med Univ, Basic Med Coll, Dept Pathophysiol, Chongqing 400016, Peoples R China; [Yang, Mengxin; Zhang, Xinyue; Zhang, Li; Yang, Yongqiang] Chongqing Med Univ, Lab Stem Cell & Tissue Engn, Chongqing 400016, Peoples R China; [Shao, Ruyue] Chongqing Med & Pharmaceut Coll, Clin Med Sch, Chongqing 400016, Peoples R China"

通信作者："Zhang, L; Yang, YQ (通讯作者)，Chongqing Med Univ, Basic Med Coll, Dept Pathophysiol, Chongqing 400016, Peoples R China.; Zhang, L; Yang, YQ (通讯作者)，Chongqing Med Univ, Lab Stem Cell & Tissue Engn, Chongqing 400016, Peoples R China."

来源：EXPERIMENTAL BIOLOGY AND MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000870448500001

JCR分区：Q3

影响因子：3.2

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Glutamine; fulminant hepatitis; inflammation; apoptosis; lipopolysaccharide; D-galactosamine

摘要："Fulminant hepatitis remains a critical health problem owing to its high mortality rate and the lack of effective therapies. An increasing number of studies have shown that glutamine supplementation provides protective benefits in inflammation-related disorders, but the pharmacological significance of glutamine in lipopolysaccharide (LPS)/D-galactosamine (D-Gal)-induced fulminant hepatitis remains unclear. In the present study, the potential effects of glutamine on LPS/D-Gal-induced fulminant hepatitis were investigated. Pretreatment with glutamine decreased plasma activities of alanine and aspartate aminotransferases, and ameliorated hepatic morphological abnormalities in LPS/D-Gal-exposed mice. Glutamine pretreatment also inhibited LPS/D-Gal-induced tumor necrosis factor alpha (TNF-alpha) and interleukin-6 (IL-6) production. In addition, glutamine pretreatment decreased the level of cleaved cysteinyl aspartate-specific proteinase 3 (caspase-3), suppressed the activities of caspase-3, caspase-8, and caspase-9, and reduced the number of cells positive for TdT-mediated dUTP nick-end labeling in LPS/D-Gal-challenged mice. Interestingly, post-treatment with glutamine also provided protective benefits against LPS/D-Gal-induced acute liver injury, although these effects were less robust than those of glutamine pre-treatment. Thus, glutamine may have potential value as a pharmacological intervention in fulminant hepatitis."

基金机构：Science and Technology Research Program of the Chongqing Municipal Education Commission [KJQN201900435]; National Natural Science Foundation of China [81871606]

基金资助正文："The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This research was supported by a grant from the Science and Technology Research Program of the Chongqing Municipal Education Commission (No. KJQN201900435) and a grant from the National Natural Science Foundation of China (No. 81871606)."