miR-135a-5p Suppresses TBK1 and Activates NRF2/TXNIP Antioxidant Pathway in LPS-Driven ALI in Mice

作者全名："Zou, Guotao; Zeng, Yiwen; Wang, Yingjuan; Luo, Yong"

作者地址："[Zou, Guotao; Zeng, Yiwen; Luo, Yong] Chongqing Med Univ, Dept Pediat, Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China; [Wang, Yingjuan] Chongqing Med Univ, Dept Emergency, Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China"

通信作者："Luo, Y (通讯作者)，Chongqing Med Univ, Dept Pediat, Yongchuan Hosp, 439 Xuanhua Rd, Chongqing 402160, Peoples R China."

来源：COMPUTATIONAL AND MATHEMATICAL METHODS IN MEDICINE

ESI学科分类：MATHEMATICS

WOS号：WOS:000874502300006

JCR分区：Q2

影响因子：2.809

年份：2022

卷号：2022

期号：　

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结束页：　

文献类型：Article

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摘要："Objective. Acute inflammation and oxidative stress are present in large numbers in patients with acute lung injury (ALI). This investigation probed miR-135a-5p/TBK1 axis within ALI together with its new therapeutic target. Methods. MLE-12 cultures were treated with lipopolysaccharide (LPS) and transfected with miR-135a-5p mimics or TBK1 vector. An ALI mouse model was also established. Analysis was done on the relationships between TBK1 and miR-135a-5p. Inflammatory components, SOD, MDA, and ROS content were all assessed. Results. Obvious inflammatory lesions were observed in lung tissues of ALI mice. Overexpression of miR-135a-5p or TBK1 knockdown remarkably decreased IL-1 beta, IL-6, and TNF-alpha serum concentrations and increased IL-10 level within lung tissues. Activated NRF2/TXNIP pathway and oxidative stress were additionally found within ALI murines, which were regulated by miR-315a-5p and TBK1. Further research revealed that miR-135a-5p negatively regulated TBK1 expression to mediate proinflammatory response and oxidative stress. Conclusion. miR135a-5p targeted TBK1 to regulate inflammatory/oxidative stress responses in ALI. Such results might bring a new potential target for ALI treatment."

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