Human amniotic mesenchymal stem cells promote endometrium regeneration in a rat model of intrauterine adhesion

作者全名："Mao, Yanhua; Yang, Yuan; Sun, Congcong; Zou, Yulong; Zhang, Yingfeng; Wu, Benyuan; Li, Changjiang; Huang, Jinglin; Zhang, Wenwen; Wang, Jia"

作者地址："[Mao, Yanhua; Sun, Congcong; Zhang, Yingfeng; Wu, Benyuan; Li, Changjiang; Huang, Jinglin; Zhang, Wenwen; Wang, Jia] Chongqing Med Univ, Dept Obstet & Gynecol, Univ Town Hosp, 55 Daxuecheng Middle Rd, Chongqing 401331, Peoples R China; [Yang, Yuan] Shanghai Jiading Maternal Child Hlth Hosp, Dept Obstet & Gynecol, Shanghai, Peoples R China; [Zou, Yulong] Chongqing Med Univ, Dept Orthoped Surg, Affiliated Hosp 2, Chongqing, Peoples R China"

通信作者："Zhang, WW; Wang, J (通讯作者)，Chongqing Med Univ, Dept Obstet & Gynecol, Univ Town Hosp, 55 Daxuecheng Middle Rd, Chongqing 401331, Peoples R China."

来源：CELL BIOLOGY INTERNATIONAL

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000876901000001

JCR分区：Q3

影响因子：3.9

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：cell differentiation; estrogen; human amniotic mesenchymal stem cells; intrauterine adhesions; regeneration; stem cell transplantation

摘要："Human amniotic transplantation has been proposed to improve the therapeutic efficacy of intrauterine adhesions (IUAs). Human amniotic mesenchymal stem stromal cells (hAMSCs) can differentiate into multiple tissue types. This study aimed to investigate the mechanism by which hAMSCs transplantation promotes endometrial regeneration. The rat models with IUA were established through mechanical and infective methods, and PKH26-labeled hAMSCs were transplanted through the tail vein (combined with/without estrogen). Under three different conditions, hAMSCs differentiated into endometrium-like cells. HE and Mason staining assays, and immunohistochemistry were used to compare the changes in rat models treated with hAMSCs and/or estrogen transplantation. To define the induction of hAMSCs to endometrium-like cells in vitro, an induction medium (cytokines, estrogen) was used to investigate the differentiation of hAMSCs into endometrium-like cells. qRT-polymerase chain reaction (PCR) and western blotting were performed to detect the differentiation of hAMSCs into endometrium-like cells. A greater number of glands, fewer endometrial fibrotic areas, and stronger expression of vascular endothelial growth factor and cytokeratin in the combined group (hAMSCs transplantation combined with estrogen) than in the other treatment groups were observed. hAMSCs could be induced into endometrium-like cells by cytokine treatment (TGF-beta 1, EGF, and PDGF-BB). Transplantation of hAMSCs is an effective alternative for endometrial regeneration after injury in rats. The differentiation protocol for hAMSCs will be useful for further studies on human endometrial regeneration."

基金机构："Chongqing Study abroad Entrepreneurship and Innovation Project [cx2020056]; Yuzhong District Science and Technology Commission Chongqing Municipality [KJQN202100460]; science and technology research program of Chongqing Education Commission of China [KJQN202100460]; Chongqing Science and Technology Commission [cstc2020jcyjmsxmX0206]; Program for Youth Innovation in Future Medicine, Chongqing Medical University [W0123]; Chongqing Education Commission [KJQN20190432]"

基金资助正文："Chongqing Study abroad Entrepreneurship and Innovation Project, Grant/Award Number: cx2020056; the Yuzhong District Science and Technology Commission Chongqing Municipality, Grant/Award Number: KJQN202100460; science and technology research program of Chongqing Education Commission of China, Grant/Award Number: KJQN202100460; Chongqing Science and Technology Commission, Grant/Award Number: cstc2020jcyjmsxmX0206; Program for Youth Innovation in Future Medicine, Chongqing Medical University, Grant/Award Number: W0123; Chongqing Education Commission, Grant/Award Number: KJQN20190432"