rM2e-APly protein immunization induces protection against influenza viruses and its co-infection with Streptococcus pneumoniae in mice

作者全名："Guo, Ting; Xiao, Jiangming; Li, Lian; Xu, Wenlong; Yuan, Yuan; Yin, Yibing; Zhang, Xuemei"

作者地址："[Guo, Ting; Xiao, Jiangming; Li, Lian; Xu, Wenlong; Yuan, Yuan; Yin, Yibing; Zhang, Xuemei] Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med, Minist Educ, Chongqing 400016, Peoples R China"

通信作者："Zhang, XM (通讯作者)，Chongqing Med Univ, Dept Lab Med, Key Lab Diagnost Med, Minist Educ, Chongqing 400016, Peoples R China."

来源：MOLECULAR IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000880854000004

JCR分区：Q3

影响因子：3.6

年份：2022

卷号：152

期号：　

开始页：86

结束页：96

文献类型：Article

关键词：Influenza a virus; Influenza matrix protein 2 ectodomain; Universal influenza vaccine; Streptococcus pneumoniae; Co -infection

摘要："Current seasonal influenza A virus (IAV) vaccines only protect against specific virus and require annual recon-stitution to accommodate the viral mutations. A universal influenza vaccination that protects against all IAV strains is urgently needed. The influenza matrix protein 2 ectodomain (M2e) is a potential universal IAV vaccine candidate, but it has a low immunogenicity. AA146Ply was proved to be an effective protein adjuvant. Therefore, AA146Ply was used as an adjuvant of M2e in this study to evaluate its protective effect against IAV-related infection. Herein, a novel rM2e protein containing multiple M2e originated from different species of IAV was constructed and expressed in Escherichia coli (E. coli). Meanwhile, we also constructed and expressed the rM2e-APly protein in E. coli. These proteins were administered intramuscularly to BALB/c mice. rM2e-APly protein induces higher levels of humoral and cellular responses compared with their comprising protein mixture or rM2e alone. rM2e-APly protein enhances the survival rate, reduces viral loads and inflammatory response in lung challenged with PR8. The serum induced by rM2e-APly protein can protect against PR8 by passive immunity and cross-react with multiple M2e peptides derived from different IAV subtypes. After IAV and Streptococcus pneu-moniae (S. pneumoniae) co-infection, rM2e-Ply protein can improve survival, lower viral and bacterial burdens, and diminish inflammatory response in the lungs. These results demonstrate that rM2e-APly protein can significantly protect against influenza virus, IAV and S. pneumoniae co-infection, indicating that rM2e-APly protein has the potential to become a universal influenza vaccine."

基金机构：National Natural Science Founda- tion of China; [81571622]

基金资助正文：This study was supported by the National Natural Science Founda- tion of China (No. 81571622) .