Protective Effects of Lactobacillus reuteri on Intestinal Barrier Function in a Mouse Model of Neonatal Necrotizing Enterocolitis

作者全名："Lai, Zhuoli; Gong, Fang"

作者地址："[Lai, Zhuoli; Gong, Fang] Chongqing Med Univ, Yongchuan Hosp, Dept Pediat, Chongqing, Peoples R China; [Lai, Zhuoli] Childrens Hosp Yongchuan Dist, Dept Pediat, Chongqing, Peoples R China; [Gong, Fang] Chongqing Med Univ, Yongchuan Hosp, Dept Pediat, 439 Xuanhua Rd, Chongqing 402160, Peoples R China"

通信作者："Gong, F (通讯作者)，Chongqing Med Univ, Yongchuan Hosp, Dept Pediat, 439 Xuanhua Rd, Chongqing 402160, Peoples R China."

来源：AMERICAN JOURNAL OF PERINATOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000884598100002

JCR分区：Q3

影响因子：2

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：neonatal necrotizing enterocolitis; Lactobacillus reuteri DSM 17938; oxidative damage; intestinal barrier; inflammation

摘要："Objective The intestinal mucosal and immune barriers play considerable roles in the pathogenesis of necrotizing enterocolitis (NEC). The present research was designed to assess the protective effects of Lactobacillus reuteri (LR) DSM 17938 (LR 17938) on the intestinal barriers and its beneficial effects on inflammation in a neonatal mouse model of NEC. Study Design Overall, 7-day-old 75 C57BL/6 neonatal mice were separated into three groups ( n = 25) as follows: (1) control, (2) NEC, and (3) NEC + LR17938 (LR group). NEC mice were administered a hypertonic feeding formula and subjected to asphyxia and hypothermia. Hematoxylin and eosin (HE) staining and pathological scores were used to assess the pathological changes in the intestine. Oxidative stress was evaluated based on the levels of superoxide dismutase (SOD) and malondialdehyde (MDA). Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1 beta levels were detected to assess inflammation. Gut permeability levels, bacterial translocation, and the levels of secretory idioglobulin A (sIgA), beta-defensin, and tight junction (TJ) proteins were detected to evaluate gut mucosal and immune barrier function, and gut microbial diversity was detected to assess the composition of the gut flora. Results LR 17938 administration decreased the NEC-induced increase in intestinal scores, mortality rate, gut damage, the MDA level, and TNF-alpha and IL-1 beta expressions. Besides, LR 17938 improved the survival rate of NEC mice. Moreover, LR 17938 administration improved gut permeability levels, SOD activity and the bacterial translocation, ameliorated the expression of TJ proteins, and improved the gut microbiota compared with those of NEC mice. Conclusion LR 17938 reduced intestinal inflammation and played a protective role in a neonatal animal model of NEC, possibly by regulating oxidative stress and exerting a protective effect on the gut mucosal and immune barriers."

基金机构：Chongqing Education Commission; [KJQN201900420]

基金资助正文："This study was supported by a grant from the Chongqing Education Commission, project no.: KJQN201900420."