Sp4 Regulates PTTG1IP Gene Transcription and Expression

作者全名："Dai, Xi; Luo, Shuyue; Guo, Shipeng; Zhou, Weihui; Song, Weihong"

作者地址："[Dai, Xi; Luo, Shuyue; Guo, Shipeng; Zhou, Weihui; Song, Weihong] Chongqing Med Univ, Childrens Hosp, Chongqing Key Lab Translat Med Res Cognit Dev & Le, Natl Clin Res Ctr Child Hlth & Disorders,Minist Ed, Chongqing, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Inst Aging, Sch Mental Hlth, Zhejiang Prov Clin Res Ctr Mental Disorders,Key La, Wenzhou, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Affiliated Hosp 2, Kangning Hosp, Wenzhou, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou, Zhejiang, Peoples R China; [Zhou, Weihui] Chongqing Med Univ, Childrens Hosp, Chongqing Key Lab Translat Med Res Cognit Dev & Le, Natl Clin Res Ctr Child Hlth & Disorders,Minist Ed, Chongqing 400010, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Inst Aging, Sch Mental Hlth, Zhejiang Prov Clin Res Ctr Mental Disorders,Key La, Wenzhou 325000, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Kangning Hosp, Affiliated Hosp 2, Wenzhou 325000, Zhejiang, Peoples R China; [Song, Weihong] Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China"

通信作者："Zhou, WH (通讯作者)，Chongqing Med Univ, Childrens Hosp, Chongqing Key Lab Translat Med Res Cognit Dev & Le, Natl Clin Res Ctr Child Hlth & Disorders,Minist Ed, Chongqing 400010, Peoples R China.; Song, WH (通讯作者)，Wenzhou Med Univ, Inst Aging, Sch Mental Hlth, Zhejiang Prov Clin Res Ctr Mental Disorders,Key La, Wenzhou 325000, Zhejiang, Peoples R China.; Song, WH (通讯作者)，Wenzhou Med Univ, Kangning Hosp, Affiliated Hosp 2, Wenzhou 325000, Zhejiang, Peoples R China.; Song, WH (通讯作者)，Wenzhou Med Univ, Yuying Childrens Hosp, Wenzhou 325000, Zhejiang, Peoples R China."

来源：DNA AND CELL BIOLOGY

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000885651200001

JCR分区：Q2

影响因子：3.1

年份：2022

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：PTTG1IP; Sp4; promoter; transcription; expression

摘要："Pituitary tumor-transforming gene 1 protein (PTTG)-interacting protein, also known as PTTG-binding factor (PBF), is encoded by a proto-oncogene PTTG1IP. PBF has been identified through its interaction with PTTG. Similar to PTTG, PBF has been implicated in the etiology of several tumors, including pituitary, thyroid, and breast cancer. PBF can induce the translocation of PTTG into the nucleus, and then lead to tumorigenesis. Studies have shown that PBF plays a vital and complex role in increasing tumor development. However, the transcriptional regulation of PTTG1IP gene remains undefined. In this study, we have cloned a 467-bp fragment of the 5 ' flanking region of the human PTTG1IP gene and identified the region (-212 to +7 bp) necessary for PTTG1IP gene promoter activity by luciferase assay. Electrophoretic mobility shift assay revealed PTTG1IP gene promoter containing Sp4 response elements. Overexpression of Sp4 increased PTTG1IP gene transcription and expression in HeLa cells. Our study demonstrates that Sp4 regulates PTTG1IP gene transcription and expression."

基金机构：Graduate Student Research Innovation Project of Chongqing; National NaturalScience Foundation of China (NSFC) [81571388]

基金资助正文："This study was supported by the Graduate Student Research Innovation Project of Chongqing, National NaturalScience Foundation of China (NSFC) grant 81571388(W.Z.)"