"Electroacupuncture regulates macrophage, neutrophil, and oral microbiota to alleviate alveolar bone loss and inflammation in experimental ligature-induced periodontitis"

作者全名："Liu, Kehao; Yang, Liangjie; Wang, Xu; Huang, Qi; Tuerhong, Kamoran; Yang, Mingcong; Zhang, Rong; Li, Yuzhou; Yang, Sheng"

作者地址："[Liu, Kehao; Yang, Liangjie; Wang, Xu; Huang, Qi; Tuerhong, Kamoran; Yang, Mingcong; Li, Yuzhou; Yang, Sheng] Chongqing Med Univ, Coll Stomatol, Dept Prosthodont, Chongqing, Peoples R China; [Zhang, Rong] Peking Univ, Neurosci Res Inst, Beijing, Peoples R China; [Zhang, Rong] Peking Univ, Sch Basic Med Sci, Dept Neurobiol, Beijing, Peoples R China; [Zhang, Rong] Peking Univ, Sch Basic Med Sci, Dept Integrat Chinese & Western Med, Beijing, Peoples R China; [Zhang, Rong] Minist Educ, Key Lab Neurosci, Beijing, Peoples R China; [Zhang, Rong] Minist Hlth, Key Lab Neurosci, Beijing, Peoples R China; [Zhang, Rong] Autism Res Ctr Peking Univ Hlth Sci Ctr, Beijing, Peoples R China; [Li, Yuzhou; Yang, Sheng] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China; [Li, Yuzhou; Yang, Sheng] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Li, Yuzhou; Yang, Sheng] Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China"

通信作者："Zhang, R (通讯作者)，Peking Univ, Neurosci Res Inst, Beijing, Peoples R China.; Li, YZ; Yang, S (通讯作者)，Chongqing Med Univ, Coll Stomatol, Chongqing 401147, Peoples R China."

来源：JOURNAL OF CLINICAL PERIODONTOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000891153100001

JCR分区：Q1

影响因子：5.8

年份：2023

卷号：50

期号：3

开始页：368

结束页：379

文献类型：Article

关键词：electroacupuncture stimulation; myeloid cells; periodontal microbiome; periodontitis

摘要："Aim: Electroacupuncture (EA) regulates distant body physiology through somatic sensory autonomic reflexes, balances the microbiome, and can promote the release of immune cells into bloodstream, thereby inhibiting severe systemic inflammation. This makes it possible to use EA as an integrated treatment for periodontitis. Materials and Methods: In this study, EA was applied to the ST36 acupoints in a ligature-induced periodontitis (LIP) mouse model. Then the effects of EA on periodontal myeloid cells, cytokines, and the microbiome were comprehensively analysed using flow cytometry, quantitative Polymerase Chain Reaction (PCR), and 16 S sequencing. Results: Results demonstrated that EA could significantly relieve periodontal bone resorption. EA also suppressed the infiltration of macrophages and neutrophils, reduced gene expression of the pro-inflammatory cytokines IL-1 beta, IL-6, IL-17 and TNF-alpha, and increased expression of the anti-inflammatory factors IL-4 and IL-10 in periodontal tissues. Moreover, composition of the periodontal microbiome was regulated by EA, finding that complex of microbiota, including supragingival Veillonella, subgingival Streptococcus, and subgingival Erysipelatoclostridium, were significantly reduced. Meanwhile, nitrate and nitrate-related activities of subgingival microbiota were reversed. Network analysis revealed close relationships among Veillonella, Streptococcus, and Bacteroides. Conclusions: Our study indicates that EA can effectively alleviate inflammation and bone resorption in LIP mice, potentially via the regulation of myeloid cells, cytokines, and periodontal microbiome."

基金机构："National Natural Science Foundation of China [81901057, 82001103, 82170936, 82171010]; Natural Science Foundation of Chongqing [cstc2019jcyj-bshX0005, cstc2021jcyj-jqX0028]; Chongqing Yuzhong District Science and Technology Project; CQMU Program for Youth Innovation in Future Medicine [W0079]"

基金资助正文："National Natural Science Foundation of China,Grant/Award Numbers: 81901057, 82001103, 82170936, 82171010; Natural Science Foundation of Chongqing, Grant/Award Numbers: cstc2019jcyj-bshX0005,cstc2021jcyj-jqX0028; Chongqing Yuzhong District Science and Technology Project;CQMU Program for Youth Innovation in Future Medicine, Grant/Award Number:W0079"