Simultaneous determination of sex hormones and bile acids in rat plasma using a liquid chromatography-tandem mass spectrometry method

作者全名："Li, Yun; Zhang, Dan; Mo, Yan; Zeng, Teng; Wu, Tongzhi; Liu, Lingqiao; Zhang, Hua; Chen, Chang"

作者地址："[Li, Yun; Mo, Yan; Zeng, Teng] Chongqing Med Univ, Clin Coll 1, Chongqing 400016, Peoples R China; [Zhang, Dan; Zhang, Hua] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing 400016, Peoples R China; [Wu, Tongzhi] Univ Adelaide, Adelaide Med Sch, Adelaide 5005, Australia; [Wu, Tongzhi] Univ Adelaide, Ctr Res Excellence CRE Translating Nutr Sci Good H, Adelaide 5005, Australia; [Liu, Lingqiao] Chongqing Three Gorges Med Coll, Sch Pharm, Chongqing 404120, Peoples R China; [Chen, Chang] Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China"

通信作者："Zhang, H (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing 400016, Peoples R China.; Chen, C (通讯作者)，Chongqing Med Univ, Inst Life Sci, Chongqing 400016, Peoples R China."

来源：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

ESI学科分类：PHARMACOLOGY & TOXICOLOGY

WOS号：WOS:000892537300001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：223

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Sex hormone; Bile acid; Rat; Pregnant; Mass spectrometry

摘要："Endogenous steroids, including sex hormones and bile acids, are a group of essential compounds with various biological functions. In this study, we developed an LC-MS method that simultaneously measures 14 sex hormones and metabolites (SH) and 32 bile acids (BA) in rat plasma. Multiple innovative approaches were applied to increase the sensitivity and specificity, including optimization of the mobile phases, gradients, and dynamic multiple reaction monitoring (DMRM) transitions. The method was validated and applied on plasma samples from pregnant rats before and 0.5 h after oral glucose tolerance test (OGTT) at gestational days 0.5 and 18.5. Results showed that the method was applicable, and 9 SH and 30 BA were measurable in the samples. In summary, this method is applicable in studies on SH and BA in rat plasma, and may also be used on other matrix and species."

基金机构：National Natural Science Foundation of China [82071671]; Scientific Research and Innovative Experiment Program for Undergraduate Students at the Chongqing Medical University [SRIEP202159]; "SKY" project of Life Science Institute of Chongqing Medical University [SKY2020]

基金资助正文："This work was supported by the National Natural Science Foundation of China (No. 82071671), the Scientific Research and Innovative Experiment Program for Undergraduate Students at the Chongqing Medical University (No. SRIEP202159) and the ""SKY"" project of Life Science Institute of Chongqing Medical University (No. SKY2020)."