Respiratory Microbiome Profile of Pediatric Pulmonary Hypertension Patients Associated With Congenital Heart Disease

作者全名："Wang, Ting; Xing, Yue; Peng, Bingming; Yang, Kai; Zhang, Chenting; Chen, Yuqin; Geng, Gang; Li, Qubei; Fu, Jian; Li, Mi; Luo, Zhengxiu; Fu, Zhou; Wang, Jian"

作者地址："[Wang, Ting; Peng, Bingming; Geng, Gang; Li, Qubei; Luo, Zhengxiu; Fu, Zhou] Chongqing Med Univ, Childrens Hosp, Dept Resp, Chongqing, Peoples R China; [Fu, Jian] Chongqing Med Univ, Childrens Hosp, Dept thorac & Cardiac Surg, Chongqing, Peoples R China; [Li, Mi] Chongqing Med Univ, Dept Cardiovasc Med Childrens Hosp, Chongqing, Peoples R China; [Xing, Yue; Yang, Kai; Zhang, Chenting; Chen, Yuqin; Wang, Jian] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ,Key Lab Child Dev & Disorders, Chongqing, Peoples R China; [Xing, Yue; Yang, Kai; Zhang, Chenting; Chen, Yuqin; Wang, Jian] Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Hlth, Natl Clin Res Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou, Peoples R China; [Wang, Jian] Guangzhou Med Univ, Affiliated Hosp 1, Natl Clin Res Ctr Resp Dis, State Key Lab Resp Dis, Guangdong 510030, Peoples R China; [Fu, Zhou] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Dept Resp, Chongqing 400014, Peoples R China"

通信作者："Wang, J (通讯作者)，Guangzhou Med Univ, Affiliated Hosp 1, Natl Clin Res Ctr Resp Dis, State Key Lab Resp Dis, Guangdong 510030, Peoples R China.; Fu, Z (通讯作者)，Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Dept Resp, Chongqing 400014, Peoples R China."

来源：HYPERTENSION

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000894180700023

JCR分区：Q1

影响因子：6.9

年份：2023

卷号：80

期号：1

开始页：214

结束页：226

文献类型：Article

关键词：16s rRNA; children; congenital heart disease; microbiota; pulmonary hypertension

摘要："Background:Pulmonary hypertension (PH) associated with congenital heart disease (CHD) is the most common type of PH in pediatric patients. The airway microbiome profile in CHD-PH patients remains rarely studied. Methods:A total of 158 children were recruited for collection of oropharyngeal swabs to sequence the 16S ribosomal RNA (16S rRNA) V3-V4 region of respiratory microbiome, to establish a correlation between these bacterial groups and echocardiography indicators in CHD-PH patients. Results:Bacterial alpha- and beta-diversity of the airway microbiome indicated a significantly lower richness in the CHD-PH group and compositional differences associated with the specific taxa and their relative abundances in the upper respiratory tract. Principal coordinate analysis showed that the pharynx microbiota composition in the CHD-PH group varied from that in the CHD or control group. The linear discriminant analysis effect size also highlighted an increased presence of Streptococcus and Rothia in pediatric CHD-PH patients. Comparison of microbial composition between pediatric and adult PH patients showed significant differences and separation of microbiota. The correlation between bacterial abundance and transthoracic echocardiography indexes in CHD-associated PH indicated that different groups of microbiomes may be related to different PH grades. Conclusions:In summary, our study reported the systematic definition and divergent profile of the upper respiratory tract microbiota in pediatric PH patients, CHD and reference subjects, as well as between pediatric and adult PH patients."

基金机构："National Natural Science Foundation of China [82000034, 82120108001, 82170069, 81970057, 8180005, 82170065]; Chongqing Science and Technology Commission [cxtc2020jcyj-msxmX0261]"

基金资助正文："This work was partially supported by grants from the National Natural Science Foundation of China (82000034, 82120108001, 82170069, 81970057, 8180005, 82170065) and the Chongqing Science and Technology Commission (cxtc2020jcyj-msxmX0261)."