Distribution of endotoxin in maternal and fetal body with intrahepatic cholestasis of pregnancy and its association with adverse fetal outcome

作者全名："Huang, Xiaomei; Lei, Lei; Feng, Fan; Shao, Yong"

作者地址："[Huang, Xiaomei; Lei, Lei; Feng, Fan; Shao, Yong] Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing, Peoples R China; [Lei, Lei] Peoples Hosp Kaizhou Dist, Dept Obstet & Gynecol, Chongqing, Peoples R China; [Lei, Lei] Chongqing Jiangbei Dist Maternal & Child Hlth Hosp, Dept Obstet & Gynecol, Chongqing, Peoples R China"

通信作者："Shao, Y (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Obstet & Gynecol, Chongqing, Peoples R China."

来源：BMC PREGNANCY AND CHILDBIRTH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000895944100001

JCR分区：Q2

影响因子：3.1

年份：2022

卷号：22

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Endotoxin; Lipopolysaccharide; Intrahepatic cholestasis of pregnancy; Ursodeoxycholic acid; Resveratrol

摘要："Background: Intrahepatic cholestasis of pregnancy is a pregnancy-specific liver disease. In this study, we sought to explore the distribution of lipopolysaccharide in the maternal body, and its effect on the fetal body in the intrahepatic cholestasis of pregnancy mice. It provides a new sight for the clinical treatment of women with intrahepatic cholestasis of pregnancy. Methods: The serum levels of lipopolysaccharide and lipopolysaccharide binding protein in women with intrahepatic cholestasis of pregnancy were analyzed. To assess the association between lipopolysaccharide levels and adverse fetal outcomes, ursodeoxycholic acid, resveratrol, and phosphatidylinositol-3-kinase inhibitor were employed in intrahepatic cholestasis of pregnancy mice, and we studied the fluorescence intensity and distribution of lipopolysaccharide in mice with intrahepatic cholestasis of pregnancy. Results: Our data indicated significantly elevated levels of lipopolysaccharide and lipopolysaccharide binding protein in women with intrahepatic cholestasis of pregnancy. In vivo fluorescence imaging revealed that the intensity of lipopolysaccharide in mice with intrahepatic cholestasis of pregnancy was higher than that in the control group, and decreased after ursodeoxycholic and resveratrol treatment. The fluorescence intensity analysis indicated that lipopolysaccharide levels in maternal liver, placenta, fetal brain and fetal liver were significantly higher in the intrahepatic cholestasis pregnancy mice group than in the control group. Conclusions: This study provided evidence of endotoxin distribution in maternal liver, placenta, fetal liver and fetal brain in mice with intrahepatic cholestasis of pregnancy. Ursodeoxycholic acid and resveratrol treatment effectively reduced lipopolysaccharide levels in pregnant mice with intrahepatic cholestasis of pregnancy."

基金机构：National Natural Science Foundation of China; Chongqing Health Planning Commission [81471473]; [2019ZDXM055]

基金资助正文："This work is funded by National Natural Science Foundation of China (No: 81471473) and Chongqing Health Planning Commission project (No. 2019ZDXM055).These fundings supported the design of the study and collection, analysis, and interpretation of the data."