A Pilot Study of Aptamer-Conjugated Silk Ligament with MSCs Recruitment Ability for ACL Reconstruction

作者全名："Wang, Xin; Huang, Yang; Song, Xiongbo; Guo, Lin; Chen, Guangxing; Yang, Liu; Chen, Cheng; Gong, Xiaoyuan"

作者地址："[Wang, Xin; Huang, Yang; Song, Xiongbo; Guo, Lin; Chen, Guangxing; Yang, Liu; Gong, Xiaoyuan] Third Mil Med Univ, Army Med Univ, Southwest Hosp, Ctr Joint Surg, Chongqing, Peoples R China; [Chen, Cheng] Chongqing Med Univ, Chongqing, Peoples R China; [Gong, Xiaoyuan] Third Mil Med Univ, Southwest Hosp, Ctr Joint Surg, 30 Gaotanyan Main St, Chongqing 400038, Peoples R China"

通信作者："Gong, XY (通讯作者)，Third Mil Med Univ, Southwest Hosp, Ctr Joint Surg, 30 Gaotanyan Main St, Chongqing 400038, Peoples R China."

来源：JOURNAL OF NATURAL FIBERS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000897823800001

JCR分区：Q1

影响因子：2.8

年份：2023

卷号：20

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Anterior cruciate ligament; silk fiber; aptamer; mesenchymal stem cell; tensile strength; regeneration

摘要："Anterior cruciate ligament (ACL) rupture is one of the most common knee injuries. Due to the limited self-healing capacity, complex structure, and mechanical environment, the regeneration of ACL remains a challenge. To achieve fast recovery and long-term ACL regeneration, biodegradable scaffolds with excellent initial mechanical support and cell recruitment ability have gained increasing research interest. The aptamer-functionalized silk fiber was fabricated by embedding silk fiber into morpholinoethanesulfonic/1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride, N-hydroxysuccinimide buffer with aptamer 19s (Apt19s) and characterized in vitro. The clinically size-matched silk ligament was braided utilizing commercial methodology (polyethylene terephthalate ligament), and the tensile strength was analyzed. A total of 18 rabbits were used to test the early mesenchymal stem cell (MSC) recruitment of Apt19s-functionalized silk ligaments. Macroscopic analysis and hematoxylin and eosin and immunohistochemistry stainings were applied at 3, 5, and 7 days post-implantation. The ultimate strength of silk fiber was lower than that of PET fibers. However, the prepared silk ligament had comparable mechanical strength to the native ACL. In vitro, the Apt19s-functionalized fiber recruited bone marrow-derived MSCs. In vivo, Apt19s-functionalized silk ligament enhanced early MSCs recruitment after implantation in comparison with the aptamer-free ligament. The Apt19s-functionalized scaffold silk ligament exhibited comparable mechanical strength with native ACL and enhanced endogenous MSC homing."

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