Coordination-induced self-assembly based carbon dot dendrimers as efficient signal labels for electrochemiluminescent immunosensor construction

作者全名："Lin, Zi; Cheng, Wenqian; Liu, Changjin; Zhao, Min; Ding, Shijia; Deng, Zhongliang"

作者地址："[Lin, Zi; Deng, Zhongliang] Chongqing Med Univ, Affiliated Hosp 2, Dept Orthoped, Chongqing 400010, Peoples R China; [Cheng, Wenqian; Zhao, Min; Ding, Shijia] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Liu, Changjin] Fifth Peoples Hosp Chongqing, Dept Lab Med, Chongqing 400062, Peoples R China"

通信作者："Deng, ZL (通讯作者)，Chongqing Med Univ, Affiliated Hosp 2, Dept Orthoped, Chongqing 400010, Peoples R China.; Zhao, M (通讯作者)，Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：TALANTA

ESI学科分类：CHEMISTRY

WOS号：WOS:000899547300003

JCR分区：Q1

影响因子：5.6

年份：2023

卷号：254

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Carbon dots; Electrochemiluminescence; Coordination -induced self -assembly; Immunosensor; Bone alkaline phosphatase

摘要："In this work, an ""on-off"" electrochemiluminescent immunosensor for bone alkaline phosphatase (BALP) detection was constructed based on the carbon dot dendrimers (CD DRs) as signal labels and Pt nanoparticles function-alized Ni-phenolic coordination spheres (Pt@Ni-PCS) as quenching labels. The prepared CD DRs with low cytotoxicity were synthesized via coordination-induced self-assembly of carboxyl groups-rich N-doped carbon dots and zirconium oxygen clusters, of which the ECL efficiency was 2-fold enhancement than that of discrete N -doped carbon dots. Benefiting from the efficient quenching effect of Pt@Ni-PCS toward Zr-CD DRs/triethyl-amine-based ECL system, the prepared immunoassay for BALP detection exhibited a broad linear range with 1 pg/mL to 50 ng/mL and a low limit of detection of 24.9 fg/mL. Importantly, this highlights coordination-induced self-assembly as a tool for carbon dots enrichment to construct efficient ECL nanocomposites, which conceptu-alizes a pathway to expand the application of carbon dots in clinical ECL analysis technology."

基金机构：National Natural Science Foundation of China; Natural Science Foundation Proj- ect of CQ CSTC; [82272431]; [21804015]; [cstc2018jcyjAX0206]

基金资助正文："This work was supported by the National Natural Science Foundation of China (82272431, 21804015) , the Natural Science Foundation Proj- ect of CQ CSTC (cstc2018jcyjAX0206) ."