miR-29a-5p regulates the malignant biological process of liver cancer cells through ARID2 regulation of EMT

作者全名："Li, Wenke; Jiang, Yourang; Pan, Qi; Yang, Guang"

作者地址："[Li, Wenke] Chongqing Med Univ, Dept Hepatol Surg, Yongchuan Hosp, Chongqing, Peoples R China; [Jiang, Yourang; Pan, Qi] Chongqing Hosp Tradit Chinese Med, Dept Dermatol, Chongqing, Peoples R China; [Yang, Guang] Chongqing Med Univ, Dept Urol Surg, Affiliated Hosp 1, Chongqing, Peoples R China"

通信作者："Yang, G (通讯作者)，Chongqing Med Univ, Dept Urol Surg, Affiliated Hosp 1, Chongqing, Peoples R China."

来源：ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000899743300001

JCR分区：Q3

影响因子：2.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：liver cancer; metastasis; proliferation; invasion; miR-29a-5p

摘要："Background. Liver cancer, the vast majority of cases being hepatocellular carcinoma (HCC), is now the most malignant tumor in the world. Recurrence and metastasis remain the major obstacles on the way to the suc- cessful treatment of HCC. In recent years, the vital function of microRNAs (miRNAs) in human health and disease have been demonstrated. Large amounts of evidence demonstrate that miRNAs play an important role in the occurrence and progression of HCC.Objectives. To find new targets for improving the early diagnosis, treatment and clinical prognosis of liver cancer. Materials and methods. We used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) to analyze the expression of miR-29a-5p. A cell counting kit-8 (CCK-8) assay was used to measure the prolif- eration of liver cancer cells. Wound healing and transwell assays were used to detect migration and invasion in vitro. Western blot was used to detect the expression of the related protein.Results. The miR-29a-5p was identified as a tumor-related miRNA. It is upregulated in HCC. The overex- pression of miR-29a-5p contributes to the proliferation, invasion and metastasis of HCC cells. Furthermore, the downregulation of miR-29a-5p inhibited the growth, migration and invasion of HCC cells in vitro. Subse- quently, we used bioinformatics methods to predict that AT-rich interaction domain 2 (ARID2) is the down- stream target gene of miR-29a-5p. The downregulation of ARID2 could reverse the tumor suppressive effect caused by the knockdown of miR-29a-5p. Similarly, the epithelial-mesenchymal transition (EMT)-related protein epithelial marker E-cadherin expression increased and the mesenchymal marker Vimentin decreased when we downregulated the expression of miR-29a-5p. Interestingly, the knockdown of ARID2 could reverse this phenomenon.Conclusions. Our study demonstrated that miRNA-29a-5p was overexpressed in HCC cells. It promotes the progression of HCC by targeting ARID2 in an EMT manner."

基金机构："National Natural Science Foundation of China; Natural Science Foundation of Chongqing, China; [81904218]; [cstc2019jcyj-msxmX0095]"

基金资助正文："Funding sources The study was supported by the National Natural Science Foundation of China (grant No. 81904218 to Q.P.) and Natural Science Foundation of Chongqing, China (grant No. cstc2019jcyj-msxmX0095 to Q.P.) ."