LncRNA RPPH1 acts as a molecular sponge for miR-122 to regulate Wnt1/beta-catenin signaling in hepatocellular carcinoma

作者全名："Zhou, Jian; Shi, Kun; Huang, Weifeng; Zhang, Yuke; Chen, Qingsong; Mou, Tong; Wu, Zhongjun; Wei, Xufu"

作者地址："[Zhou, Jian] Peoples Hosp Rongchang Dist, Dept Hepatobiliary Surg, Chongqing, Peoples R China; [Zhou, Jian; Shi, Kun; Huang, Weifeng; Zhang, Yuke; Mou, Tong; Wu, Zhongjun; Wei, Xufu] First Affiliated Hosp Chongqing Med Univ, Dept Hepatobiliary Surg, Chongqing, Peoples R China; [Chen, Qingsong] Chongqing Univ Cent Hosp, Dept Traumatol, Chongqing, Peoples R China"

通信作者："Wu, ZJ; Wei, XF (通讯作者)，First Affiliated Hosp Chongqing Med Univ, Dept Hepatobiliary Surg, Chongqing, Peoples R China."

来源：INTERNATIONAL JOURNAL OF MEDICAL SCIENCES

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000899976500003

JCR分区：Q1

影响因子：3.2

年份：2023

卷号：20

期号：1

开始页：23

结束页：34

文献类型：Article

关键词：lncRNA RPPH1; miR-122; competing endogenous RNA; Wnt signaling; hepatocellular carcinoma

摘要："This study aimed to explore the role of lncRNA RPPH1 in hepatocellular carcinoma. The expression of RPPH1 and miR-122 was determined by Real-time PCR. Cell proliferation and colony formation assays were employed to monitor cell growth in vitro. Wound healing and Transwell assays were applied to detect cell migration and invasion. A dual-luciferase reporter assay was used to verify the interaction between RPPH1 and miR-122. The in vivo function of RPPH1 was illustrated by xenograft tumor models. The results showed that the expression of RPPH1 was markedly upregulated in human HCC specimens and cell lines compared to normal controls. However, the trend of miR-122 was the opposite. RPPH1 facilitates the proliferation, migration, and invasion of HCC cells and synchronously suppresses cell apoptosis. The dual-luciferase assay confirmed the relationship between RPPH1 and miR-122. Rescue experiments showed that RPPH1 acted as a competing endogenous RNA (ceRNA) by sponging miR-122 in HCC cells. Moreover, RPPH1 positively regulated the expression of Wnt1 and its downstream targets through miR-122. Our study demonstrates for the first time that RPPH1 promotes HCC progression via the miR-122/Wnt1/beta-catenin axis, which may represent a valuable therapeutic target for patients with HCC."

基金机构："National Natural Science Foundation of China [81703063]; Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) [2021MSXM056]; Natural Science Foundation of Chongqing, China [cstc2021jcyj-msxmX0200]"

基金资助正文："This study was supported by the National Natural Science Foundation of China (No. 81703063) , the Chongqing medical scientific research project (Joint project of Chongqing Health Commission and Science and Technology Bureau) (No. 2021MSXM056) and the Natural Science Foundation of Chongqing, China (No. cstc2021jcyj-msxmX0200) ."