Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter strains in China: a multicenter genomic study

作者全名："Zhu, Zhichen; Xie, Xiaofang; Yu, Hua; Jia, Wei; Shan, Bin; Huang, Bin; Qu, Fen; Niu, Siqiang; Lv, Jinnan; Gao, Qizhao; Qian, Feinan; Tian, Xiangxiang; Zhai, Yaxuan; Wen, Yicheng; Yang, Chengcheng; Zhu, Jie; Tang, Yiwei; Chen, Liang; Du, Hong"

作者地址："[Zhu, Zhichen; Xie, Xiaofang; Lv, Jinnan; Gao, Qizhao; Qian, Feinan; Tian, Xiangxiang; Zhai, Yaxuan; Wen, Yicheng; Yang, Chengcheng; Zhu, Jie; Du, Hong] Soochow Univ, Dept Clin Lab, Affiliated Hosp 2, Suzhou, Jiangsu, Peoples R China; [Yu, Hua] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Dept Lab Med, Chengdu, Peoples R China; [Yu, Hua] Univ Elect Sci & Technol China, Sichuan Prov Peoples Hosp, Sichuan Prov Key Lab Human Dis Gene Study, Chengdu, Peoples R China; [Jia, Wei] Ningxia Med Univ, Ctr Med Lab, Gen Hosp, Yinchuan, Ningxia, Peoples R China; [Shan, Bin] Kunming Med Univ, Dept Lab Med, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China; [Huang, Bin] Sun Yat Sen Univ, Dept Lab Med, Affiliated Hosp 1, Guangzhou, Peoples R China; [Qu, Fen] Aviat Gen Hosp, Lab Med Ctr, Beijing, Peoples R China; [Niu, Siqiang] Chongqing Med Univ, Dept Lab Med, Affiliated Hosp 1, Chongqing, Peoples R China; [Tang, Yiwei] Mem Sloan Kettering Canc Ctr, Dept Lab Med, 1275 York Ave, New York, NY 10021 USA; [Tang, Yiwei] Danaher Diagnost Platform Cepheid China, Dept Med Affairs, New York, NY USA; [Chen, Liang] Hackensack Meridian Hlth, Ctr Discovery & Innovat, Nutley, NJ USA; [Chen, Liang] Seton Hall Univ, Hackensack Meridian Sch Med, Nutley, NJ USA"

通信作者："Du, H (通讯作者)，Soochow Univ, Dept Clin Lab, Affiliated Hosp 2, Suzhou, Jiangsu, Peoples R China."

来源：EMERGING MICROBES & INFECTIONS

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000900312000001

JCR分区：Q1

影响因子：8.4

年份：2023

卷号：12

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Enterobacter; bla(NDM); IncX3; ST171; ST116; carbapenemase

摘要："Epidemiological characteristics and molecular features of carbapenem-resistant Enterobacter (CR-Ent) species remain unclear in China. In this study, we performed a genomic study on 92 isolates from Enterobacter-caused infections from a multicenter study in China. Whole genome sequencing (WGS) was used to determine the genome sequence of 92 non-duplicated CR-Ent strains collected from multiple tertiary health centres. The precise species of Enterobacter strains were identified by average nucleotide identity (ANI) and in silico DNA-DNA hybridization (isDDH). Molecular features of high-risk CR-Ent sequence type (ST) lineages and carbapenemase-encoding plasmids were determined. The result revealed that the most common human-source CR-Ent species in China was E. xiangfangensis (66/92, 71.93%), and the proportion of carbapenemase-producing Enterobacter (CP-Ent) in CR-Ent was high (72/92, 78.26%) in comparison to other global regions. Furthermore, ST171 and ST116 E. xiangfangensis were the major lineages of CP-Ent strains, and ST171 E. xiangfangensis was more likely to cause infections in older patients. Genomic analysis also highlighted the likelihood of intra-hospital/inter-hospital clonal transmission of ST171 and ST116 E. xiangfangensis. In addition, the bla(NDM)-harbouring IncX3-type plasmid was identified as the prevalent carbapenemase-encoding plasmid carried by CR-Ent strains, and was experimentally confirmed to be able to self-transfer with high frequency. This study detailed the genomic and clinical characteristics of CR-Ent in China in the form of multicenter for the first time. The high risk of carbapenemase-producing ST171 and ST116 E. xiangfangensis, and the bla(NDM)-harbouring IncX3-type plasmid were detected and emphasized."

基金机构：Science Foundation of Jiangsu Province Health Department; Science Foundation of Suzhou Health Department [ZDB2020014]; Science and Technology Program of Suzhou [LCZX202106]; Science and Technology Program of Suzhou [SZS201715]; Discipline Construction Program of the Second Affiliated Hospital of Soochow University [SLT201934]; Natural Science Fund of China [XKTJ-TD202001]; [82002204]

基金资助正文：This study was supported by the Science Foundation of Jiangsu Province Health Department (ZDB2020014); the Science Foundation of Suzhou Health Department (LCZX202106); the Science and Technology Program of Suzhou (SZS201715); the Science and Technology Program of Suzhou (SLT201934); the Discipline Construction Program of the Second Affiliated Hospital of Soochow University (XKTJ-TD202001) and the Natural Science Fund of China (82002204).