"Investigation of Cell Adhesion and Cell Viability of the Endothelial and Fibroblast Cells on Electrospun PCL, PLGA and Coaxial Scaffolds for Production of Tissue Engineered Blood Vessel"

作者全名："Bazgir, Morteza; Saeinasab, Morvarid; Zhang, Wei; Zhang, Ximu; Tsui, Ka Min; Sarvestani, Abolfazl Maasoumi; Nawaz, Subhaan; Coates, Phil; Youseffi, Mansour; Elies, Jacobo; Sefat, Farshid"

作者地址："[Bazgir, Morteza; Tsui, Ka Min; Sarvestani, Abolfazl Maasoumi; Nawaz, Subhaan; Youseffi, Mansour; Sefat, Farshid] Univ Bradford, Sch Engn, Dept Biomed & Elect Engn, Bradford BD7 1DP, England; [Saeinasab, Morvarid] Ferdowsi Univ Mashhad, Fac Sci, Dept Biol, Mashhad 9177948974, Iran; [Zhang, Wei] Sichuan Univ, Polymer Res Inst, State Key Lab Polymer Mat Engn, Chengdu 610065, Peoples R China; [Zhang, Wei] Sichuan Univ, Adv Polymer Mat Res Ctr, Shishi 362700, Peoples R China; [Zhang, Ximu] Chongqing Med Univ, Stomatol Hosp, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing 401174, Peoples R China; [Zhang, Ximu] Chongqing Med Univ, Stomatol Hosp, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing 401174, Peoples R China; [Coates, Phil; Sefat, Farshid] Univ Bradford, Interdisciplinary Res Ctr Polymer Sci & Technol, Polymer IRC, Bradford BD7 1DP, England; [Elies, Jacobo] Univ Bradford, Fac Life Sci, Sch Pharm & Med Sci, Bradford BD7 1DP, England"

通信作者："Sefat, F (通讯作者)，Univ Bradford, Sch Engn, Dept Biomed & Elect Engn, Bradford BD7 1DP, England.; Sefat, F (通讯作者)，Univ Bradford, Interdisciplinary Res Ctr Polymer Sci & Technol, Polymer IRC, Bradford BD7 1DP, England.; Elies, J (通讯作者)，Univ Bradford, Fac Life Sci, Sch Pharm & Med Sci, Bradford BD7 1DP, England."

来源：JOURNAL OF FUNCTIONAL BIOMATERIALS

ESI学科分类：　

WOS号：WOS:000900862600001

JCR分区：Q2

影响因子：4.8

年份：2022

卷号：13

期号：4

开始页：　

结束页：　

文献类型：Article

关键词：tissue engineering; electrospinning; polycaprolactone (PCL); poly (lactic-co-glycolic acid) (PLGA); cell viability; cell proliferation; human umbilical vein cells (HUVEC); human vascular fibroblast cells (HVF)

摘要："Endothelialization of artificial scaffolds is considered an effective strategy for increasing the efficiency of vascular transplantation. This study aimed to compare the biophysical/biocompatible properties of three different biodegradable fibrous scaffolds: Poly (epsilon-caprolactone) (PCL) alone, Poly Lactic-co-Glycolic Acid (PLGA) alone (both processed using Spraybase (R) electrospinning machine), and Coaxial scaffold where the fiber core and sheath was made of PCL and PLGA, respectively. Scaffold structural morphology was assessed by scanning electron microscope and tensile testing was used to investigate the scaffold tension resistance over time. Biocompatibility studies were carried out with human umbilical vein endothelial cells (HUVEC) and human vascular fibroblasts (HVF) for which cell viability (and cell proliferation over a 4-day period) and cell adhesion to the scaffolds were assessed by cytotoxicity assays and confocal microscopy, respectively. Our results showed that all biodegradable polymeric scaffolds are a reliable host to adhere and promote proliferation in HUVEC and HVF cells. In particular, PLGA membranes performed much better adhesion and enhanced cell proliferation compared to control in the absence of polymers. In addition, we demonstrate here that these biodegradable membranes present improved mechanical properties to construct potential tissue-engineered vascular graft."

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