High Sensitivity and Wide Linearity LC-MS/MS Method for Oxylipin Quantification in Multiple Biological Samples

作者全名："Fu, Xian; Yin, Hou-Hua; Wu, Ming-Jun; He, Xin; Jiang, Qing; Zhang, Ling-Tong; Liu, Jun-Yan"

作者地址："[Fu, Xian; Yin, Hou-Hua; He, Xin; Jiang, Qing; Zhang, Ling-Tong; Liu, Jun-Yan] Chongqing Med Univ, Inst Life Sci, Ctr Novel Target & Therapeut Intervent, Chongqing, Peoples R China; [Wu, Ming-Jun] Chongqing Med Univ, Inst Life Sci, Chongqing, Peoples R China"

通信作者："Liu, JY (通讯作者)，Chongqing Med Univ, Inst Life Sci, Ctr Novel Target & Therapeut Intervent, Chongqing, Peoples R China."

来源：JOURNAL OF LIPID RESEARCH

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000901592500006

JCR分区：Q1

影响因子：6.5

年份：2022

卷号：63

期号：12

开始页：　

结束页：　

文献类型：Article

关键词：biological regulators; liquid chromatography-tandem mass spectrometry; quantitative profiling; lower limits of quantitation; reduced sample; consumption; lipoxins; resolvins; plasma samples; quantitative sensitivity; linear range

摘要："Oxylipins are important biological regulators that have received extensive research attention. Due to the extremely low concentrations, large concentration variations, and high structural similarity of many oxylipins, the quantitative analysis of oxylipins in biological samples is always a great challenge. Here, we developed a liquid chromatography-tandem mass spectrometry-based method with high sensitivity, wide linearity, and acceptable resolution for quantitative profiling of oxylipins in multiple biological samples. A total of 104 oxylipins, some with a high risk of detection crosstalk, were well separated on a 150 mm column over 20 min. The method showed high sensitivity with lower limits of quantitation for 87 oxylipins, reaching 0.05-0.5 pg. Unexpectedly, we found that the linear range for 16, 18, and 17 oxylipins reached 10,000, 20,000, and 40,000 folds, respectively. Due to the high sensitivity, while reducing sample consumption to below half the volume of previous methods, 74, 78, and 59 low-abundance oxylipins, among which some were difficult to detect like lipoxins and resolvins, were well quantified in the tested mouse plasma, mouse liver, and human plasma samples, respectively. Additionally, we determined that analytes with multifarious concentrations of over a 1,000-fold difference could be well quantified simultaneously due to the wide linearity. In conclusion, most likely due to the instrumental advancement, this method effectively improves the quantitative sensitivity and linear range over existing methods, which will facilitate and advance the study of the physiological and pathophysiological functions of oxylipins."

基金机构：Chongqing Postdoctoral Sustentation Fund [cstc2021jcyj-bshX0164]; National Natural Science Foundation of China [82273408]; Chongqing Education Committee University Innovation Research Group Grant [CXQT21016]

基金资助正文："This work was cosupported by the Chongqing Postdoctoral Sustentation Fund (cstc2021jcyj-bshX0164), National Natural Science Foundation of China (82273408), and Chongqing Education Committee University Innovation Research Group Grant (CXQT21016)."