Nickel-Doped Microfluidic Chip for Rapid and Efficient Immunomagnetic Separation and Detection of Breast Cancer Cell-Derived Exosomes

作者全名："Fang, Huiying; Liu, Mei; Jiang, Wei"

作者地址："[Fang, Huiying; Liu, Mei] Chongqing Univ, Dept Breast Dis, Chongqing Key Lab Intelligent Oncol Breast Canc iC, Canc Hosp, 181 Hanyu Rd, Chongqing 400030, Peoples R China; [Jiang, Wei] Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing 400010, Peoples R China"

通信作者："Jiang, W (通讯作者)，Chongqing Med Univ, Dept Radiol, Affiliated Hosp 2, 74 Linjiang Rd, Chongqing 400010, Peoples R China."

来源：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000901959100001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Microfluidic chip; Exosomes; Exosome separation; Exosome detection; Immunoaffinity

摘要："Breast cancer cell-derived exosomes have high potential as biomarkers for continuous biopsies and longitudinal monitoring in breast cancer. However, it is extremely difficult to separate exosomes with high recovery and high purity from complex media, such as urine, plasma, saliva and cell culture supernatants. Here, we designed a flexible and simple microfluidic chip for exosome separation. The capture zone of the chip is a three-dimensional structure of interlaced cylinders doped with nickel powder. Exosomes were separated from cell culture supernatant by the immunomagnetic separation method in continuous flow mode and were detected by fluorescence imaging with high sensitivity. The chip achieved a high exosome recovery rate (> 74%) and purity (> 67%) at an injection rate of 3.6 mL/h. Thus, this chip was demonstrated to be a cutting-edge platform for the separation and detection of exosomes. It could also be applied to separate and detect other types of exosomes, microbubbles and cells."

基金机构："Natural Science Foundation of Chongqing, China [cst-c2020jcyj-msxmX0367]; Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau) [2022MSXM136]"

基金资助正文："This research was supported by the Natural Science Foundation of Chongqing, China (No. cst-c2020jcyj-msxmX0367) and the Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau, No. 2022MSXM136)."