Periodontal Ligament Stem Cell-Derived Extracellular Vesicles Enhance Tension-Induced Osteogenesis

作者全名："Xu, Jingchen; Lin, Yao; Tian, Mi; Li, Xinyi; Yin, Yuanyuan; Li, Qiming; Li, Ziyu; Zhou, Jialiang; Jiang, Xiaoge; Li, Yulin; Chen, Song"

作者地址："[Xu, Jingchen; Lin, Yao; Tian, Mi; Li, Xinyi; Li, Qiming; Li, Ziyu; Zhou, Jialiang; Jiang, Xiaoge; Li, Yulin; Chen, Song] Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Dept Orthodont,State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China; [Yin, Yuanyuan] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing Municipal Key Lab Oral Biomed Engn Highe, Stomatol Hosp, Chongqing 401147, Peoples R China"

通信作者："Chen, S (通讯作者)，Sichuan Univ, West China Hosp Stomatol, Natl Clin Res Ctr Oral Dis, Dept Orthodont,State Key Lab Oral Dis, Chengdu 610041, Sichuan, Peoples R China."

来源：ACS BIOMATERIALS SCIENCE & ENGINEERING

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000901996300001

JCR分区：Q2

影响因子：5.4

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：periodontal ligament stem cells; small extracellular vesicles; osteogenesis; bone remodeling; microRNA

摘要："Tension-induced osteogenesis has great significance in maintaining bone homeostasis and ensuring the efficiency and stability of orthodontic treatment. Recently, extracellular vesicles (EVs) have shown great potential in regulating bone remodeling. Here, we aimed to explore the effects of periodontal ligament stem cell (PDLSC)-derived EVs on tension-induced osteogenesis and the potential mechanism. PDLSC-derived EVs were extracted by ultracentrifugation. In vitro, PDLSC-derived EVs of 10 mu g/mL significantly improved the proliferation of MC3T3-E1 cells and enhanced the osteogenic differentiation of osteoblasts under a tensile strain of 2000 u epsilon. Next, a mouse model of orthodontic tooth movement (OTM) was established and treated with subperiosteal injection of PDLSC-derived EVs (1 mg/kg) on the tension side. The results showed that treatment with PDLSC-derived EVs effectively enhanced OTM and promoted osteogenesis on the tension side, including increasing trabecular bone parameters and promoting the expression of osteogenic-related biomarkers (OCN and OPN). More interestingly, we identified several mechano-sensitive miRNAs enriched in PDLSC-derived EVs by high-throughput miRNA sequencing. Bioinformatics analysis indicated that they were related to various osteogenesis-related signaling pathways. Therefore, PDLSC-derived EVs could improve the efficiency of OTM by enhancing tension-induced osteogenesis of osteoblasts. Our study may provide potential evidence for the promoting effects of PDLSC-derived EVs on osteogenesis and offer new insights into the development of treatment strategies for enhancing osteogenesis in orthodontic treatment and other metabolic bone diseases."

基金机构：National Natural Science Foundation of China; Fund ofScience and Technology Department of Sichuan Province; [81671021]; [2022YFS0127]

基金资助正文：ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China (number: 81671021) and the Fund ofScience and Technology Department of Sichuan Province. (2022YFS0127) .