Determination of 23 Amino Acids and Amines in Plasma and Cortex of C57BL/6 Mice with HPLC-FLD

作者全名："Zhang, Jing; Yang, Junqing; Luo, Ying; Li, Jiansha; Gong, Tao; Huang, Suqiong; Xiong, Yan; Jiang, Xinhui"

作者地址："[Zhang, Jing; Gong, Tao; Huang, Suqiong; Xiong, Yan; Jiang, Xinhui] Chongqing Med Univ, Chongqing 400016, Peoples R China; [Yang, Junqing; Luo, Ying] Chongqing Med Univ, Key Lab Pharmacol Chongqing, Chongqing 400016, Peoples R China; [Li, Jiansha] Jiangsu Hengrui Pharmaceut Co Ltd, Chongqing 400042, Peoples R China"

通信作者："Jiang, XH (通讯作者)，Chongqing Med Univ, Chongqing 400016, Peoples R China."

来源：JOURNAL OF CHROMATOGRAPHIC SCIENCE

ESI学科分类：CHEMISTRY

WOS号：WOS:000902128300001

JCR分区：Q3

影响因子：1.5

年份：2023

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文献类型：Article; Early Access

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摘要："Using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as a pre-column derivatization reagent, optimized derivatization and chromatography parameters, a simple high-performance liquid chromatography fluorescence detector (HPLC-FLD) method was developed and validated to determine 23 related amines in plasma and cortex of C57BL/6 mice with cerebral ischemia-reperfusion injury. The prepared samples were separated on a ZORBAX SB-C18 column (4.6 mm x 250 mm, 5 mu m) with 60% acetonitrile (ACN) and 20 mM sodium acetate solution (pH adjusted to 5.0 by phosphoric acid). All analytes achieved good separation within 1.2 h at a flow rate of 1 mL/min. The limits of detection and limits of detection quantitation of the method were ranged from (0.1-9.2) to (0.3-30.6) ng/mL, respectively. The analytical method was apt for simultaneously determining 23 amino acids in plasma and cortex. Our results revealed that the relevant amino acids were significantly altered (P < 0.05) in C57BL/6 mice."

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