Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of necrotizing enterocolitis: A prospective study

作者全名："Liu, Xiao-Chen; Li, Lu-Quan; Ling, Ke-Ran; Guo, Lu; Hu, Xiao-Yu; Li, Chun"

作者地址："[Liu, Xiao-Chen; Li, Lu-Quan; Ling, Ke-Ran; Guo, Lu; Hu, Xiao-Yu; Li, Chun] Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Neonatal Diag & Treatment Ctr,Minist Educ,Key Lab, Chongqing, Peoples R China"

通信作者："Li, C (通讯作者)，Chongqing Med Univ, Childrens Hosp, Natl Clin Res Ctr Child Hlth & Disorders, Neonatal Diag & Treatment Ctr,Minist Educ,Key Lab, Chongqing, Peoples R China."

来源：FRONTIERS IN PEDIATRICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000903017200001

JCR分区：Q2

影响因子：2.6

年份：2022

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：human beta-defensin 2; claudin-3; biomarker; deterioration; necrotizing enterocolitis

摘要："Background and purpose: Necrotizing enterocolitis (NEC) is a critical gastrointestinal disease. We aim to explore the value of fecal human beta-defensin 2 (HBD-2), Claudin-3, high-mobility group box-1 protein (HMGB-1), and resistin-like molecule beta (Relm beta) as well as some laboratory metrics to predict the deterioration of NEC. Methods: Infants diagnosed with NEC at Stage II were enrolled in our study. Those who progressed to Stage III were included in the Stage III group and the rest were included in the Stage II group. Clinical data and laboratory metrics of the infants were collected. Fecal samples of HBD2, HMGB-1, Claudin-3, and Relm beta collected during their enrollment were determined by using enzyme-linked immunosorbent assay (ELISA) kits. Student's t-test, the Mann-Whitney U test, the chi-square test, receiver operating characteristic (ROC), and logistic regression analysis were performed. Results: Sixty infants diagnosed with NEC at Stage II were enrolled in our study, with 27 in the Stage III group (n = 27) and 33 in the Stage II group (n = 33). Although many of these NEC cases were late preterm and term infants, the infants in the Stage III group had a lower gestational age (P < 0.05). The incidence of gestational diabetes mellitus, peritonitis, intestinal adhesion, and sepsis was higher and more infants in the Stage III group underwent surgeries (P < 0.05). The levels of HBD-2 and Claudin-3 were higher and neutrophil count was lower in the Stage III group than in the Stage II Group, and the area under the curve (AUC) was 0.754, 0,755, and 0.666, respectively (P < 0.05). HBD-2 >= 1649.02 ng/g and Claudin-3 >= 2488.71 pg/g were included in the multivariate stepwise logistic regression analysis (P < 0.05), and the AUC of the model was 0.805 (95% CI: 0.688-0.922). Conclusion: Fecal HBD-2 and Claudin-3 may be potential biomarkers to predict the deterioration of NEC from Stage II to Stage III."

基金机构：Natural Science Foundation of Chongqing municipality; Joint Medical Research Project of Chongqing Science and Technology Commission; [cstc2021jcyj-msxmX0063]; [2022MSXM039]

基金资助正文：Funding We acknowledge the support from the Natural Science Foundation of Chongqing municipality (cstc2021jcyj-msxmX0063) and the Joint Medical Research Project of Chongqing Science and Technology Commission (2022MSXM039).