Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice

作者全名："Zhao, Tianxin; Shen, Lianju; Ye, Xin; Bai, Gaochen; Liao, Chen; Chen, Zhicong; Peng, Tianwen; Li, Xiaomin; Kang, Xiangjin; An, Geng"

作者地址："[Zhao, Tianxin; Ye, Xin; Liao, Chen; Chen, Zhicong; Peng, Tianwen; Li, Xiaomin; Kang, Xiangjin; An, Geng] Guangzhou Med Univ, Affiliated Hosp 3, Ctr Reprod Med, Dept Obstet & Gynecol,Key Lab Major Obstet Dis Gua, Guangzhou, Peoples R China; [Zhao, Tianxin; Bai, Gaochen] Guangzhou Med Univ, Guangzhou Women & Childrens Med Ctr, Guangdong Prov Clin Res Ctr Child Hlth, Dept Urol, Guangzhou, Guangdong, Peoples R China; [Shen, Lianju] Chongqing Med Univ, Childrens Hosp, Pediat Res Inst, Chongqing, Peoples R China"

通信作者："An, G (通讯作者)，Guangzhou Med Univ, Affiliated Hosp 3, Ctr Reprod Med, Dept Obstet & Gynecol,Key Lab Major Obstet Dis Gua, Guangzhou, Peoples R China."

来源：JOURNAL OF HAZARDOUS MATERIALS

ESI学科分类：ENGINEERING

WOS号：WOS:000903719600002

JCR分区：Q1

影响因子：12.2

年份：2023

卷号：445

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Microplastics; Prenatal and postnatal exposure; Testis; Hippo; Immune reaction

摘要："Polystyrene microplastics (PS-MPs) can threaten human health, especially male fertility. However, most existing studies have focused on the adulthood stage of male reproduction toxicity caused by relatively short-term PS-MP exposure. This study aimed to investigate the toxic effect of PS-MPs on testicular development and reproductive function upon prenatal and postnatal exposure. Pregnant mice and their offspring were exposed to 0, 0.5 mg/L, 5 mg/L, and 50 mg/L PS-MPs through their daily drinking water from gestational day 1 to postnatal day (PND) 35 or PND70. We found that PS-MP exposure induced testis development disorder by PND35 and spermatogenesis dysfunction by PND70. By combining RNA sequencing results and bioinformatics analysis, the hormone -mediated signaling pathway, G1/S transition of the mitotic cell cycle, coregulation of androgen receptor ac-tivity, and Hippo signaling pathway were shown to be involved in testis development on PND35. The meiotic cell cycle, regulation of the immune effector process, neutrophil degranulation, and inflammation mediated by chemokine and cytokine signaling pathways were associated with disturbed spermatogenesis on PND70. These findings show that prenatal and postnatal exposure to PS-MPs resulted in testis development disorder and male subfertility, which may be regulated by the Hippo signaling pathway and involve an immune reaction."

基金机构："National Key Research and Development Plan [2019YFE0109500]; National Natural Science Foundation of China [82171589, 82203709]; Guangdong Basic and Applied Basic Research Foundation [2022A1515012576, 2020A1515110796, 2021A1515010774, 2019A1515011439]; Guangzhou Health Science and Technology Project [20201A011093]; Translational Fund for Academician LinHe New Medicine [2021HLKY03]; Research Fund for Clinical Doctor of Guangzhou Women and Children's Medical Center"

基金资助正文："This study was supported by the National Key Research and Development Plan [Grant number 2019YFE0109500]; the National Natural Science Foundation of China [Grant number 82171589, 82203709]; the Guangdong Basic and Applied Basic Research Foundation [Grant number 2022A1515012576, 2020A1515110796, 2021A1515010774, 2019A1515011439]; the Guangzhou Health Science and Technology Project [Grant number 20201A011093]; the Translational Fund for Academician LinHe New Medicine [Grant number 2021HLKY03]; and the Research Fund for Clinical Doctor of Guangzhou Women and Children's Medical Center."