Alphalisa immunoassay enabled centrifugal microfluidic system for "one-step" detection of pepsinogen in whole blood

作者全名："Shi, Yuxing; Guo, Jiuchuan; Guo, Jinhong"

作者地址："[Shi, Yuxing; Guo, Jiuchuan] Univ Elect Sci & Technol China, Sch Automat Engn, Chengdu 611731, Peoples R China; [Guo, Jiuchuan; Guo, Jinhong] Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China; [Guo, Jinhong] Shanghai Jiao Tong Univ, Sch Sensing Sci & Engn, Shanghai, Peoples R China"

通信作者："Guo, JH (通讯作者)，Chongqing Med Univ, Coll Lab Med, MOE Key Lab Lab Med Diagnost, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：SENSORS AND ACTUATORS B-CHEMICAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000904397400005

JCR分区：Q1

影响因子：8

年份：2023

卷号：376

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Centrifugal microfluidic; One-step detection; Alphalisa immunoassay; Pepsinogen quantification

摘要："In recent years, gastric cancer has received extensive attention and prevention. To achieve rapid and highly sensitive detection of gastric function index without blood pretreatment, in this study, for the first time, we investigated an intelligent system combining centrifugal microfluidic and Alphalisa immunoassay for early detection of pepsinogen. Based on the ""interconnected-collecting chambers collinear with rotational center"" (ICCRC) structure, the system could automatically separate whole blood and perform immune detection using Alphalisa to achieve quantitative detection of pepsinogen I (PG I) and PG II. The whole detection process took place in ""one-step"" in less than 12 min. The plasma separation efficiency reached 99 %, and limit of detection values of 0.75 ng/mL for PG I and 0.85 ng/mL for PG II were obtained. The results indicated that the portable system could detect pepsinogen with high sensitivity and accuracy, providing a great potential for highly inte-grated on-chip point-of-care testing in future."

基金机构：National Natural Sci-ence Foundation of China; [62122017]

基金资助正文：Acknowledgement The authors thank the financial support from National Natural Sci-ence Foundation of China (62122017) .