LncRNA DNAJC3-AS1 promotes the biological functions of papillary thyroid carcinoma via regulating the microRNA-27a-3p/CCBE1 axis

作者全名："Ni, Tiangen; Li, Yongyong; Guo, Dan; Tan, Ling; Xiao, Zhesi; Shi, Yanjie"

作者地址："[Ni, Tiangen; Guo, Dan; Tan, Ling; Xiao, Zhesi] Chongqing Med Univ, Affiliated Hosp 2, Dept Breast & Thyroid Surg, Chongqing, Peoples R China; [Li, Yongyong] Chongqing Med Univ, Affiliated Hosp 2, Dept Geriatr, Chongqing, Peoples R China; [Shi, Yanjie] Univ Chinese Acad Sci, Chongqing Renji Hosp, Chongqing Peoples Hosp 5, Dept Otolaryngol Head & Neck Surg, Chongqing, Peoples R China; [Shi, Yanjie] Univ Chinese Acad Sci, Chongqing Renji Hosp, Chongqing Peoples Hosp 5, Dept Otolaryngol Head & Neck Surg, 24 Renji Rd, Chongqing 401120, Peoples R China"

通信作者："Shi, YJ (通讯作者)，Univ Chinese Acad Sci, Chongqing Renji Hosp, Chongqing Peoples Hosp 5, Dept Otolaryngol Head & Neck Surg, 24 Renji Rd, Chongqing 401120, Peoples R China."

来源：CELL BIOLOGY INTERNATIONAL

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000905200200001

JCR分区：Q3

影响因子：3.3

年份：2023

卷号：47

期号：3

开始页：539

结束页：547

文献类型：Article

关键词：cell tumorigenicity; collagen and calcium-binding EGF domain-containing protein 1; long noncoding RNA DNAJC3-AS1; malignant behavior; microRNA-27a-3p; papillary thyroid carcinoma

摘要："Long noncoding RNA DNAJC3-AS1 (lncRNA DNAJC3-AS1) has been probed in many studies, while the regulatory mechanism of DNAJC3-AS1 on papillary thyroid carcinoma (PTC) via regulating microRNA (miR)-27a-3p remains inadequate. This research aims to depict the role of DNAJC3-AS1, miR-27a-3p, collagen, and calcium-binding EGF domain-containing protein 1 (CCBE1) on PTC development. DNAJC3-AS1, miR-27a-3p, and CCBE1 expression levels in PTC tissues and adjacent normal tissues were tested. The relation of DNAJC3-AS1, miR-27a-3p, and CCBE1 was analyzed. DNAJC3-AS1 and miR-27a-3p and CCBE1-related oligonucleotides were transfected into IHH-4 cells to investigate their role in PTC development. Cell tumorigenicity was detected by in vivo assay. DNAJC3-AS1 and CCBE1 expressed highly and miR-27a-3p expressed lowly in PTC. Downregulation of DNAJC3-AS1, upregulating miR-27a-3p or downregulating CCBE1 impaired the malignant behaviors of IHH-4 cells. Depletion of miR-27a-3p reversed the DNAJC3-AS1 suppression-induced phenotypic inhibition of IHH-4 cells. DNAJC3-AS1 bound to miR-27a-3p and CCBE1 as a target of miR-27a-3p. Our study highlights that DNAJC3-AS1 inhibits miR-27a-3p to promote CCBE1 expression, thereby facilitating PTC development. This study affords distinguished therapeutic strategies and novel research directions for PTC treatment."

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