Sulfhydryl functionalized hyaluronic acid hydrogels attenuate cyclophosphamide-induced bladder injury

作者全名："Qiu, Heping; Li, Jinjin; Huang, Yuandi; Shen, Chongxing; Dai, Linyong; Su, Qiaoling; Zhi, Yi; Fang, Qiang; Shi, Chunmeng; Li, Weibing"

作者地址："[Qiu, Heping; Li, Jinjin; Huang, Yuandi; Shen, Chongxing; Dai, Linyong; Su, Qiaoling; Zhi, Yi; Fang, Qiang; Li, Weibing] Chongqing Med Univ, Affiliated Hosp 3, Chongqing 401120, Peoples R China; [Shi, Chunmeng] Army Med Univ, Mil Med Univ 3, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China"

通信作者："Li, WB (通讯作者)，Chongqing Med Univ, Affiliated Hosp 3, Chongqing 401120, Peoples R China.; Shi, CM (通讯作者)，Army Med Univ, Mil Med Univ 3, Inst Rocket Force Med, State Key Lab Trauma Burns & Combined Injury, Chongqing 400038, Peoples R China."

来源：BIOMEDICAL MATERIALS

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000905623100001

JCR分区：Q2

影响因子：3.9

年份：2023

卷号：18

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：hyaluronic acid; hydrogels; sulfhydryl functionalization; hemorrhagic cystitis; cyclophosphamide

摘要："Clinical management of cyclophosphamide (CYP) results in numerous side effects including hemorrhagic cystitis (HC), which is characterized by inflammation and oxidative stress damage. Intravesical hyaluronic acid (HA) supplementation, a therapeutic method to restore barrier function of bladder, avoid the stimulation of metabolic toxicants on bladder and reduce inflammatory response, has shown good results in acute or chronic bladder diseases. However, there are unmet medical needs for the treatment of HC to temporarily restore bladder barrier and reduce inflammation. Herein, sulfhydryl functionalized HA (HA-SH) and dimethyl sulfoxide (DMSO) were used to prepared a hydrogel system for optimizing the treatment of HC. We systematically evaluated the physicochemical of hydrogels and their roles in a rat model of CYP-induced HC. The prepared hydrogels exhibited outstanding gel forming properties, injectability, and biosafety. Swelling and retention studies showed that hydrogels were stable and could prolong the residence time of HA in the bladder. Histopathology and vascular permeability studies indicated that the hydrogels significantly attenuated bladder injury caused by CYP administration. Moreover, the hydrogels also showed excellent anti-inflammation and anti-oxidation properties. In conclusion, these data suggest that intravesical instillation of HA-SH/DMSO hydrogels reduces CYP-induced bladder toxicity and this work provides a new strategy for the prevention and early treatment of HC."

基金机构："Key Program of National Natural Science Foundation of China [82030056]; Research project of the Third Affiliated Hospital of Chongqing Medical University [KY08026]; Science and technology project of Yubei District, Chongqing [2021-NS-42]; Intramural Research Project Grants [2018-JCJQ-ZQ-001, AWS21J003]"

基金资助正文："This work was supported by the Intramural Research Project Grants (2018-JCJQ-ZQ-001 and AWS21J003), the Key Program of National Natural Science Foundation of China (82030056), Research project of the Third Affiliated Hospital of Chongqing Medical University (KY08026), and Science and technology project of Yubei District, Chongqing (2021-NS-42)."