An Adhesive Bioink toward Biofabrication under Wet Conditions

作者全名："Li, Wanlu; Wang, Mian; Wang, Shiwei; Wang, Xiaoping; Avila, Alan; Kuang, Xiao; Mu, Xuan; Garciamendez, Carlos Ezio; Jiang, Zewei; Manriquez, Jennifer; Tang, Guosheng; Guo, Jie; Mille, Luis Santiago; Robledo, Juan Antonio; Wang, Di; Cheng, Feng; Li, Hongbin; Flores, Regina Sanchez; Zhao, Zhibo; Delavaux, Clement; Wang, Zixuan; Lopez, Arturo; Yi, Sili; Zhou, Cuiping; Gomez, Ameyalli; Schuurmans, Carl; Yang, Guo-Yuan; Wang, Yongting; Zhang, Xingcai; Zhang, Ximu; Zhang, Yu Shrike"

作者地址："[Li, Wanlu; Wang, Mian; Wang, Shiwei; Avila, Alan; Kuang, Xiao; Mu, Xuan; Garciamendez, Carlos Ezio; Jiang, Zewei; Manriquez, Jennifer; Tang, Guosheng; Guo, Jie; Mille, Luis Santiago; Robledo, Juan Antonio; Wang, Di; Cheng, Feng; Li, Hongbin; Flores, Regina Sanchez; Zhao, Zhibo; Delavaux, Clement; Wang, Zixuan; Lopez, Arturo; Yi, Sili; Zhou, Cuiping; Gomez, Ameyalli; Schuurmans, Carl] Harvard Med Sch, Brigham & Womens Hosp, Dept Med, Div Engn Med, Cambridge, MA 02139 USA; [Li, Wanlu; Yang, Guo-Yuan; Wang, Yongting] Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 20030, Peoples R China; [Li, Wanlu; Yang, Guo-Yuan; Wang, Yongting] Shanghai Jiao Tong Univ, Med X Res Inst, Shanghai 20030, Peoples R China; [Wang, Shiwei] Zhengzhou Univ, Natl Ctr Int Joint Res Micronano Molding Technol, Sch Mech & Safety Engn, Zhengzhou 450001, Peoples R China; [Wang, Xiaoping; Zhang, Ximu; Zhang, Yu Shrike] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Higher Educ & Stomatol Hosp, Chongqing 401174, Peoples R China; [Wang, Xiaoping; Zhang, Ximu; Zhang, Yu Shrike] Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn, Higher Educ & Stomatol Hosp, Chongqing 401174, Peoples R China; [Avila, Alan; Manriquez, Jennifer; Lopez, Arturo; Gomez, Ameyalli] Tecnol Monterrey, Biotechnol Program, Monterrey 64849, NL, Mexico; [Schuurmans, Carl] Univ Utrecht, Utrecht Inst Pharmaceut Sci UIPS, Dept Pharmaceut, Sci Life, Univ Weg 99, NL-3508 TB Utrecht, Netherlands; [Zhang, Xingcai] Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA; [Zhang, Xingcai] MIT, Sch Engn, Cambridge, MA 02139 USA"

通信作者："Zhang, XM; Zhang, YS (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Higher Educ & Stomatol Hosp, Chongqing 401174, Peoples R China.; Zhang, XM; Zhang, YS (通讯作者)，Chongqing Med Univ, Chongqing Municipal Key Lab Oral Biomed Engn, Higher Educ & Stomatol Hosp, Chongqing 401174, Peoples R China.; Zhang, XC (通讯作者)，Harvard Univ, John A Paulson Sch Engn & Appl Sci, Cambridge, MA 02138 USA.; Zhang, XC (通讯作者)，MIT, Sch Engn, Cambridge, MA 02139 USA."

来源：SMALL

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000905926600001

JCR分区：Q1

影响因子：13

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：adhesive bioink; bioprinting; Chinese giant salamanders; underwater; wound healing

摘要："Three-dimensional (3D) bioprinting is driving significant innovations in biomedicine over recent years. Under certain scenarios such as in intraoperative bioprinting, the bioinks used should exhibit not only cyto/biocompatibility but also adhesiveness in wet conditions. Herein, an adhesive bioink composed of gelatin methacryloyl, gelatin, methacrylated hyaluronic acid, and skin secretion of Andrias davidianus is designed. The bioink exhibits favorable cohesion to allow faithful extrusion bioprinting in wet conditions, while simultaneously showing good adhesion to a variety of surfaces of different chemical properties, possibly achieved through the diverse bonds presented in the bioink formulation. As such, this bioink is able to fabricate sophisticated planar and volumetric constructs using extrusion bioprinting, where the dexterity is further enhanced using ergonomic handheld bioprinters to realize in situ bioprinting. In vitro experiments reveal that cells maintain high viability; further in vivo studies demonstrate good integration and immediate injury sealing. The characteristics of the bioink indicate its potential widespread utility in extrusion bioprinting and will likely broaden the applications of bioprinting toward situations such as in situ dressing and minimally invasive tissue regeneration."

基金机构：American Heart Association [19TPA34850188]; Brigham Research Institute

基金资助正文："W.L., M.W., S.W., and X.W. contributed equally to this work. All animal studies were carried under the approval granted by the Dental School of Chongqing Medical University Animal Care and Use Committee, with the assigned approval number of CQHS-REC-2020-076. The authors gratefully acknowledge funding from the American Heart Association (19TPA34850188) and the Brigham Research Institute. The authors thank the NeuroTechnology Studio at Brigham and Women's Hospital for providing Zeiss LSM880 instrument access and consultation on data acquisition and data analysis. Ximu Z. further acknowledges the program for Youth Innovation in Future Medicine from Chongqing Medical Universtiy (W0075)."