Diversity of s(66)-Specific Promoters Contributes to Regulation of Developmental Gene Expression in Chlamydia trachomatis

作者全名："Shen, Li; Gao, Leiqiong; Zhang, Yan; Hua, Ziyu"

作者地址："[Shen, Li; Gao, Leiqiong] Louisiana State Univ, Dept Microbiol Immunol & Parasitol, Hlth Sci Ctr, New Orleans, LA 71103 USA; [Gao, Leiqiong; Zhang, Yan; Hua, Ziyu] Chongqing Med Univ, Childrens Hosp, Key Lab Child Dev & Disorders, Chongqing Key Lab Pediat,Minist Educ,Dept Neonatol, Chongqing, Peoples R China"

通信作者："Shen, L (通讯作者)，Louisiana State Univ, Dept Microbiol Immunol & Parasitol, Hlth Sci Ctr, New Orleans, LA 71103 USA."

来源：JOURNAL OF BACTERIOLOGY

ESI学科分类：MICROBIOLOGY

WOS号：WOS:000907626000001

JCR分区：Q3

影响因子：2.7

年份：2023

卷号：205

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Chlamydia trachomatis; sigma(66); RNA polymerase; promoter activity; transcription; extended-10 element; developmental cycle; Scc4; in vitro transcription; transcription reporter assay; extended-10 promoter; gene reporters; in vitro transcription assay; promoter function; sigma factor

摘要："Promoter recognition by the RNA polymerase (RNAP) holoenzyme is a key step in gene regulation. In Chlamydia trachomatis, a medically important obligate intracellular bacterium, sigma(66) allows the RNAP to initiate promoter-specific transcription throughout the chlamydial developmental cycle. Here, we investigated the intrinsic properties of sigma(66)-specific promoters with emphasis on their role in the developmental gene expression of C. trachomatis. First, we examined whether promoters that contain a 5'-T(-15)G(-14)-3' (TG) motif upstream from the -10 element appear more often than others in genes that are preferentially expressed during the early, middle, or late stages of the C. trachomatis developmental cycle. We then determined the critical genetic elements that are required for transcription initiation in vitro. We also assessed the activity of promoters in the presence of Scc4, which can directly interact with sigma(66)RNAP. Finally, we evaluated the promoter-specific dynamics during C. trachomatis infection using a reporter assay. These results reveal that the TG motif is an important determinant in certain early or late promoters. The TG promoters that have the -35 element are recognized by sigma(66)RNAP and Scc4 differently from those lacking the -35 element. Based on these properties, the sigma(66)-specific promoters can fall into three classes. Architectural diversity, behavioral plasticity, and the specific interplays between promoters and the sigma(66)RNAP likely contribute to developmental gene transcription in C. trachomatis."

基金机构：National Institutes of Allergy and Infectious Diseases; [AI146454]

基金资助正文：ACKNOWLEDGMENTS Research reported in this publication was supported by National Institutes of Allergy and Infectious Diseases grant number AI146454 to L.S. We are grateful to Ben Kelly for critical reading of the manuscript.