"Application of microfluidic chip technology to study the inhibitory effect of tetramethylpyrazine on platelet aggregation, activation, and phosphatidylserine exposure mediated by pathological high shear rate"

作者全名："Zhang, Tiancong; Liu, Ling; Huang, Xiaojing; Gao, Xuemei; Chen, Dan; Huan, Xuanrong; He, Cui; Li, Yuan"

作者地址："[Zhang, Tiancong; Liu, Ling; Huang, Xiaojing; Gao, Xuemei; Chen, Dan; Huan, Xuanrong; Li, Yuan] Chongqing Med Univ, Yong Chuan Hosp, Cent Lab, Chongqing 402160, Peoples R China; [He, Cui] Chongqing Med Univ, Yong Chuan Hosp, Dept Blood Transfus, Chongqing, Peoples R China"

通信作者："Li, Y (通讯作者)，Chongqing Med Univ, Yong Chuan Hosp, Cent Lab, Chongqing 402160, Peoples R China."

来源：BLOOD COAGULATION & FIBRINOLYSIS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000907668800008

JCR分区：Q4

影响因子：1.2

年份：2023

卷号：34

期号：1

开始页：47

结束页：60

文献类型：Article

关键词：pathological high shear; platelet; tetramethylpyrazine

摘要："ObjectiveIn order to study the antithrombotic effect and mechanism of tetramethylpyrazine (TMA).MethodsIn this study, we developed a microfluidic chip model that can mimic normal arteries and stenotic arterial vessels, and studied the inhibitory effects of TMA on platelet aggregation, activation (P-selectin, GPIIb/IIIa, monocyte-platelet aggregates) and phosphatidyl serine (PS) exposure. In addition, we also investigated the effect of TMA on ADP and ristocetin-induced platelet aggregation by turbidimetry.ResultsThe results showed that TMA significantly inhibited the platelet aggregation, activation and PS exposure induced by pathological high shear rate. Under static conditions, TMA can inhibit ADP and ristocetin-induced platelet aggregation.ConclusionThe results indicated that TMA mainly inhibited platelet aggregation, activation and PS exposure by inhibiting the binding of von Willebrand factor (vWF) to the GPIb/IX/V complex, and partially inhibited platelet aggregation through the platelet P2Y(12)-ADP receptor pathway."

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