Highly elastic and self-healing nanostructured gelatin/clay colloidal gels with osteogenic capacity for minimally invasive and customized bone regeneration

作者全名："Dou, Zhenzhen; Tang, Han; Chen, Kaiwen; Li, Dize; Ying, Qiwei; Mu, Zhixiang; An, Chuanfeng; Shao, Fei; Zhang, Yang; Zhang, Yonggang; Bai, Haoliang; Zheng, Guoshuang; Zhang, Lijun; Chen, Tao; Wang, Huanan"

作者地址："[Dou, Zhenzhen; Chen, Kaiwen; Ying, Qiwei; An, Chuanfeng; Shao, Fei; Zhang, Yonggang; Wang, Huanan] Dalian Univ Technol, Frontiers Sci Ctr Smart Mat Oriented Chem Engn, Sch Bioengn, State Key Lab Fine Chem, Dalian 116024, Peoples R China; [Tang, Han; Li, Dize; Mu, Zhixiang; Bai, Haoliang; Chen, Tao] Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing 401147, Peoples R China; [An, Chuanfeng] Chinese Univ Hong Kong, Longgang Dist Peoples Hosp Shenzhen, Cent Lab, Shenzhen 518172, Peoples R China; [An, Chuanfeng] Chinese Univ Hong Kong, Affiliated Hosp Provis 3, Shenzhen 518172, Peoples R China; [An, Chuanfeng] Shenzhen Univ, Hlth Sci Ctr, Sch Biomed Engn, Guangdong Key Lab Biomed Measurements & Ultrasound, Shenzhen 518060, Peoples R China; [Zhang, Yang] Shenzhen Univ, Hlth Sci Ctr, Dept Biomed Engn, Shenzhen 518037, Peoples R China; [Zheng, Guoshuang] Natl Local Joint Engn Lab Dev Orthoped Implant Mat, Dalian 116001, Peoples R China; [Zhang, Lijun] Dalian Eye Hosp, Liyun Zhang Peoples Hosp Dalian 3, Dalian 116024, Peoples R China"

通信作者："Wang, HA (通讯作者)，Dalian Univ Technol, Frontiers Sci Ctr Smart Mat Oriented Chem Engn, Sch Bioengn, State Key Lab Fine Chem, Dalian 116024, Peoples R China.; Chen, T (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Oral Dis & Biomed Sci, Stomatol Hosp, Chongqing 401147, Peoples R China."

来源：BIOFABRICATION

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000907778500001

JCR分区：Q1

影响因子：8.2

年份：2023

卷号：15

期号：2

开始页：　

结束页：　

文献类型：Article

关键词：3D bioprinting; colloidal gels; self-healing hydrogel; nanocomposite; bone regeneration; injectable biomaterials

摘要："Extrusible biomaterials have recently attracted increasing attention due to the desirable injectability and printability to allow minimally invasive administration and precise construction of tissue mimics. Specifically, self-healing colloidal gels are a novel class of candidate materials as injectables or printable inks considering their fascinating viscoelastic behavior and high degree of freedom on tailoring their compositional and mechanical properties. Herein, we developed a novel class of adaptable and osteogenic composite colloidal gels via electrostatic assembly of gelatin nanoparticles and nanoclay particles. These composite gels exhibited excellent injectability and printability, and remarkable mechanical properties reflected by the maximal elastic modulus reaching similar to 150 kPa combined with high self-healing efficiency, outperforming most previously reported self-healing hydrogels. Moreover, the cytocompatibility and the osteogenic capacity of the colloidal gels were demonstrated by inductive culture of MC3T3 cells seeded on the three-dimensional (3D)-printed colloidal scaffolds. Besides, the biocompatibility and biodegradability of the colloidal gels was proved in vivo by subcutaneous implantation of the 3D-printed scaffolds. Furthermore, we investigated the therapeutic capacity of the colloidal gels, either in form of injectable gels or 3D-printed bone substitutes, using rat sinus bone augmentation model or critical-sized cranial defect model. The results confirmed that the composite gels were able to adapt to the local complexity including irregular or customized defect shapes and continuous on-site mechanical stimuli, but also to realize osteointegrity with the surrounding bone tissues and eventually be replaced by newly formed bones."

基金机构：National Key Research and Development Program of China; National Natural Science Foundation of China; Fundamental Research Funds for the Central Universities; Chongqing Special Postdoctoral Science Foundation; Guangdong Provincial Basic and Applied Basic Research; Shenzhen Basic Research Program general Project; [2018YFA0703000]; [31870957]; [3207100383]; [52273102]; [DUT20YG103]; [DUT22LAB601]; [2010010005784771]; [2019A1515110415]; [JCYJ20190808152211686]; [JCYJ20190808120217133]

基金资助正文："AcknowledgmentsZ Dou and H Tang contributed equally to the current study. T Chen and H Wang designed the study, Z Dou and H Tang performed the experiments and analyzed the results. Z Dou, H Tang, T Chen and H Wang wrote the manuscript. All the other authors helped with the experiments and the data analysis. This work was supported by National Key Research and Development Program of China (No. 2018YFA0703000), National Natural Science Foundation of China (Nos. 31870957, 3207100383 and 52273102), the Fundamental Research Funds for the Central Universities (Nos. DUT20YG103 and DUT22LAB601), Chongqing Special Postdoctoral Science Foundation (No. 2010010005784771), Guangdong Provincial Basic and Applied Basic Research (2019A1515110415), and Shenzhen Basic Research Program general Project (JCYJ20190808152211686 and JCYJ20190808120217133)."