Spent culture supernatant of Streptococcus gordonii mitigates inflammation of human periodontal cells and inhibits proliferation of pathogenic oral microbes

作者全名："Shu, Yi; Upara, Chawin; Ding, Qiong; Zhu, Min; Zeng, Erliang; Banas, Jeffrey A. A.; Hong, Liu"

作者地址："[Shu, Yi] Chongqing Med Univ, Coll Stomatol, Chongqing, Peoples R China; [Shu, Yi; Upara, Chawin; Ding, Qiong; Zhu, Min; Zeng, Erliang; Banas, Jeffrey A. A.; Hong, Liu] Univ Iowa, Iowa Inst Oral Hlth Res, Coll Dent, Iowa City, IA 52242 USA; [Hong, Liu] Univ Iowa, Iowa Inst Oral Hlth Res, Coll Dent, Dept Prosthodont, N412 DSB,801 Newton Ave, Iowa City, IA 52242 USA; [Banas, Jeffrey A. A.] Univ Iowa, Iowa Inst Oral Hlth Res, Coll Dent, Dept Pediat Dent, N423 DSB,801 Newton Ave, Iowa City, IA 52242 USA"

通信作者："Hong, L (通讯作者)，Univ Iowa, Iowa Inst Oral Hlth Res, Coll Dent, Dept Prosthodont, N412 DSB,801 Newton Ave, Iowa City, IA 52242 USA.; Banas, JA (通讯作者)，Univ Iowa, Iowa Inst Oral Hlth Res, Coll Dent, Dept Pediat Dent, N423 DSB,801 Newton Ave, Iowa City, IA 52242 USA."

来源：JOURNAL OF PERIODONTOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000908115900001

JCR分区：Q1

影响因子：4.2

年份：2023

卷号：94

期号：4

开始页：575

结束页：585

文献类型：Article

关键词：culture media; conditioned; cytokines; inflammation; microbiology; Streptococcus gordonii

摘要："BackgroundThe oral commensal bacterial species Streptococcus gordonii has been reported to regulate the inflammation of oral epithelial cells stimulated by the periodontal pathogen Porphyromonas gingivalis. This study investigated the activities of S. gordonii metabolites in S. gordonii spent culture supernatants (Sg-SCS) on periodontal-related bacterial growth and periodontitis-associated inflammatory cytokines. MethodsSg-SCS was collected from S. gordonii cultures grown in Dulbecco Modified Eagle Medium and added to the growth media of representative health- and disease-related oral species: S. gordonii, Streptococcus sanguinis, Streptococcus mitis, Streptococcus oralis, P. gingivalis, Tannerella forsythia, and Treponema denticola. The Sg-SCS was also tested for its ability to regulate the expression of proinflammatory cytokines by human macrophages, epithelial cells, and gingival fibroblasts upon stimulation with P. gingivalis-derived lipopolysaccharide (Pg-LPS). ResultsSg-SCS significantly reduced transcript and protein levels of interleukin (IL)-1 beta, 6, and 8 induced by Pg-LPS stimulation in multiple types of periodontal cells. mRNA sequencing and bioinformatics analyses indicated that Sg-SCS significantly affects 10 inflammatory pathways. Additionally, Sg-SCS exhibited suppression of the growth of periodontal disease-related bacteria, including T. denticola and P. gingivalis, along with the primary plaque-colonizing species S. oralis. At a low concentration, Sg-SCS also inhibits P. gingivalis adhesion. ConclusionsThese results strongly suggest that S. gordonii-derived SCS contains metabolites that have anti-inflammatory properties and an ability to inhibit periodontitis-associated pathogenic bacteria. Further investigation will be needed to identify the individual metabolites within the Sg-SCS to develop a novel metabolite-based approach to treating and preventing periodontitis."

基金机构：National Institute of Dental and Craniofacial Research; National Institutes of Health; [R01DE026433]; [R01DE026716]

基金资助正文：ACKNOWLEDGMENTS This study was supported by the National Institute of Dental and Craniofacial Research (Grant No. R01DE026433 to Liu Hong; Grant No. R01DE026716 to Jeffrey A. Banas) of the National Institutes of Health.