A novel DNA detection using spherical identification probe and strand displacement reaction-initiated silver nanocluster switch

作者全名："Yang, Xiaoyan; Liu, Xiaoyu; Kang, Qi; Qi, Yinxiao; Du, Yumin; Xiang, Hua"

作者地址："[Yang, Xiaoyan; Kang, Qi; Qi, Yinxiao; Du, Yumin; Xiang, Hua] Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China; [Liu, Xiaoyu] Guangan People Hosp, Dept Clin Lab, Guangan, Peoples R China"

通信作者："Xiang, H (通讯作者)，Chongqing Med Univ, Sch Lab Med, Key Lab Lab Med Diagnost, Minist Educ, Chongqing 400016, Peoples R China."

来源：ANALYTICAL SCIENCES

ESI学科分类：CHEMISTRY

WOS号：WOS:000909036000001

JCR分区：Q3

影响因子：1.8

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Spherical identification probe; Toehold-mediated strand displacement reaction; DNA-templated silver nanocluster

摘要："Herein, we developed a novel fluorescent assay using spherical identification probes and toehold-mediated strand displacement reaction-initiated silver nanoclusters (AgNCs) ""on-off "" signal switch. In this strategy, the target was captured by the spherical probes to induce the activity of exonuclease III (Exo III), catalyzing the cyclic cleavage of substrates to produce a mass of trigger strands. After magnetic bead separation, the intermediates in the supernatant activated downstream toehold-mediated strand displacement reaction to change the structure of silver nanocluster templates, leading to fluorescence intensity reduction. Furthermore, it is demonstrated that the application of spherical identification probes could reduce the signal leakage and the limit of detection. In addition, AgNCs with perfect optical property were ingeniously combined to realize signal output, which reduced the cost and time of synthesis. Under the optimal conditions, the sensing method displayed a good linear range from 250 pM to 25 nM with a detectable minimum concentration of 250 pM. And the practical application potential in complex biological matrices was also evaluated. Considering these advantages, this constructed strategy opens a new path for nucleic acid detection with better performance."

基金机构："Innovative Research Funds for School of Laboratory Medicine, Chongqing Medical University"

基金资助正文："This work was financially supported by Innovative Research Funds for School of Laboratory Medicine, Chongqing Medical University"