Augmenter of Liver Regeneration Monoclonal Antibody Promotes Apoptosis of Hepatocellular Carcinoma Cells

作者全名："Huang, Li -Li; Luo, Fei-Yang; Huang, Wen-Qi; Guo, Hui; Liu, Qi; Zhang, Ling; Jin, Ai-Shun; Sun, Hang"

作者地址："[Huang, Li -Li; Zhang, Ling] Chongqing Med Univ, Affiliated Hosp 2, Dept Nephrol, Chongqing, Peoples R China; [Luo, Fei-Yang; Jin, Ai-Shun] Chongqing Med Univ, Coll Basic Med, Dept Immunol, Chongqing, Peoples R China; [Huang, Wen-Qi; Guo, Hui; Liu, Qi; Sun, Hang] Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Key Lab Mol Biol Infect Dis,Minist Educ, Chongqing, Peoples R China; [Sun, Hang] Chongqing Med Univ, Second Affiliated Hosp, Inst Viral Hepatitis, Key Lab Mol Biol Infect Dis,Minist Educ, Chongqing 400010, Peoples R China"

通信作者："Sun, H (通讯作者)，Chongqing Med Univ, Second Affiliated Hosp, Inst Viral Hepatitis, Key Lab Mol Biol Infect Dis,Minist Educ, Chongqing 400010, Peoples R China."

来源：JOURNAL OF CLINICAL AND TRANSLATIONAL HEPATOLOGY

ESI学科分类：　

WOS号：WOS:000911054200001

JCR分区：Q2

影响因子：3.1

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Hepatocellular carcinoma; Augmenter of liver regeneration; Mono-clonal antibody; Apoptosis

摘要："Background and Aims: Hepatocellular carcinoma (HCC) is one of the most common types of cancer, often resulting in death. Augmenter of liver regeneration (ALR), a widely expressed multifunctional protein, has roles in liver dis-ease. In our previous study, we reported that ALR knock-down inhibited cell proliferation and promoted cell death. However, there is no study on the roles of ALR in HCC. Methods: We used in vitro and in vivo models to inves-tigate the effects of ALR in HCC as well as its mechanism of action. We produced and characterized a human ALR-specific monoclonal antibody (mAb) and investigated the effects of the mAb in HCC cells. Results: The purified ALR-specific mAb matched the predicted molecular weight of IgG heavy and light chains. Thereafter, we used the ALR-specific mAb as a therapeutic strategy to suppress tumor growth in nude mice. Additionally, we assessed the prolif-eration and viability of three HCC cell lines, Hep G2, Huh-7, and MHC97-H, treated with the ALR-specific mAb. Com-pared with controls, tumor growth was inhibited in mice treated with the ALR-specific mAb at 5 mg/kg, as shown by hematoxylin and eosin staining and terminal deoxynu-cleotidyl transferase dUTP nick end labeling. Simultaneous treatment with the ALR-specific mAb and adriamycin pro-moted apoptosis, whereas treatment with the ALR-specific mAb alone inhibited cell proliferation. Conclusions: The ALR-specific mAb might be a novel therapy for HCC by blocking extracellular ALR."

基金机构："National Natu-ral Science Foundation of China; Postgraduate research and innovation projects of Chongqing Municipal Education Commission; [81871608,30971334]; [CYB21178]"

基金资助正文："Funding This work was supported by grants from the National Natu-ral Science Foundation of China (81871608,30971334) and Postgraduate research and innovation projects of Chongqing Municipal Education Commission (CYB21178) ."