Structural-Based Stability Enhancement of Antisense DNA Oligonucleotides

作者全名："Zeng, Jie; Fang, Liang; Jiang, Tingting; Liu, Mingchun; Li, Wei; Mao, Chengde; Lou, Shifeng; Zuo, Hua"

作者地址："[Zeng, Jie] Chongqing Med Univ, Coll Basic Med, Chongqing 400016, Peoples R China; [Fang, Liang; Lou, Shifeng] Second Affiliated Hosp Chongqing Med Univ, Dept Hematol, Chongqing 400010, Peoples R China; [Fang, Liang] Ninth Chongqing Peoples Hosp, Dept Oncol, Chongqing 400700, Peoples R China; [Jiang, Tingting; Liu, Mingchun; Zuo, Hua] Southwest Univ, Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China; [Li, Wei] Chongqing Med Univ, Yongchuan Hosp, Dept Infect Dis, Yongchuan, Chongqing 402160, Peoples R China; [Mao, Chengde] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA"

通信作者："Zuo, H (通讯作者)，Southwest Univ, Southwest Univ, Coll Pharmaceut Sci, Key Lab Luminescence Anal & Mol Sensing, Chongqing 400715, Peoples R China."

来源：MACROMOLECULAR BIOSCIENCE

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000911800600001

JCR分区：Q2

影响因子：4.6

年份：2023

卷号：23

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：antisense DNA oligonucleotide; DNA secondary structure; miRNA; miR-21; stability enhancement

摘要："Antisense DNA oligonucleotide (AS) technology is a promising approach to regulate gene expression and cellular processes. For example, ASs can be used to capture the overexpressed, oncogenic miRNAs in tumors to suppress tumor growth. Among many challenges faced by AS approach is the degradation of ASs by nucleases under physiological conditions. Elongating the AS lifespan can substantially enhance the functions of AS. The paper reports a simple strategy to increase the stability of ASs. The authors discover that the ASs degrade quickly if their ends are in unpaired, single-stranded form, but much slower if their ends are in paired duplex form. It is conceivable to integrate this strategy with other strategies (such as chemical modification of ASs backbones) to maximally increase the ASs stabilities."

基金机构："Chongqing Research Program of Basic Research and Frontier Technology, China [cstc2020jcyj-msxmX0947, cstc2021jcyj-msxmX0931, cstc2021jcyj-msxmX1033]; Chongqing Postdoctoral Science Foundation [CSTB2022NSCQ-BHX0688]; China Postdoctoral Science Foundation [2022M710556]; National Science Foundation of China [21974111, 22274135]; Yongchuan Natural Science Foundation, Chongqing (Ycstc) [2020nb0202]"

基金资助正文："J.Z. and L.F. contributed equally to this work. This work was financially supported by the Chongqing Research Program of Basic Research and Frontier Technology, China (cstc2020jcyj-msxmX0947, cstc2021jcyj-msxmX0931, cstc2021jcyj-msxmX1033), Chongqing Postdoctoral Science Foundation (CSTB2022NSCQ-BHX0688), the China Postdoctoral Science Foundation (2022M710556), the National Science Foundation of China (21974111 and 22274135), and Yongchuan Natural Science Foundation, Chongqing (Ycstc, 2020nb0202)."