A paper-based lateral flow strip assay based on enzyme-mediated pectin viscosity change for the determination of polygalacturonase activity

作者全名："Zhang, Hao; Yang, Feng-Qing"

作者地址："[Zhang, Hao] Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing 401331, Peoples R China; [Zhang, Hao] Chongqing Med Univ, Coll Pharm, Chongqing 400016, Peoples R China; [Yang, Feng-Qing] Chongqing Univ, Sch Chem & Chem Engn, Chongqing 401331, Peoples R China"

通信作者："Zhang, H (通讯作者)，Chongqing Med & Pharmaceut Coll, Chongqing Key Lab High Act Tradit Chinese Drug Del, Chongqing 401331, Peoples R China."

来源：MICROCHEMICAL JOURNAL

ESI学科分类：CHEMISTRY

WOS号：WOS:000912007300001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：186

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：Paper-based lateral flow strip sensor; Smartphone; Viscosity; Polygalacturonase; Pectin

摘要："In this study, a simple, convenient, and cost-effective paper-based lateral flow strip sensor combined with a smartphone was developed for the determination of polygalacturonase (PG) activity based on the modulation of viscosity of pectin solution, which can be visualized by the difference in the average diffusion distance on the pH indicator strips. Effects of the pectin concentration, enzymatic reaction time, the volume of diffusion liquid, and the diffusion time on the average diffusion distance were systematically investigated. For quantitative analysis of PG activity, the images of the pH indicator strips after lateral flow diffusion were collected by a smartphone and processed through Adobe Photoshop CS 5.1 software. Under the optimal conditions, the average lateral flow diffusion distance shows a good linear relationship with PG activity in the range of 0.025-0.80 U/mL, and the limit of quantification is 0.025 U/mL. Finally, the applicability of the proposed strategy was successfully verified by measuring PG activity in cucumber sample. The recoveries are in the range of 87.4%-96.7%. In short, the present study developed a convenient, low-cost, and accurate approach for on-site detecting of PG activity, especially in remote or resource-limited regions."

基金机构："Chongqing Postdoctoral Science Foundation, China; Chongqing Medical and Pharmaceutical College, China; [CSTB2022NSCQ-BHX0687]; [YGZ2021133]"

基金资助正文："Acknowledgements This work was sponsored by the Chongqing Postdoctoral Science Foundation, China (CSTB2022NSCQ-BHX0687) , and the Chongqing Medical and Pharmaceutical College, China (YGZ2021133) ."