Comparison of DNA methylation profiles of human embryos cultured in either uninterrupted or interrupted incubators

作者全名："Zhu, Ling; Zeng, Xi; Liu, Weiwei; Han, Wei; Huang, Guoning; Li, Jingyu"

作者地址："[Zhu, Ling; Zeng, Xi; Liu, Weiwei; Han, Wei; Huang, Guoning; Li, Jingyu] Chongqing Med Univ, Women & Childrens Hosp, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing, Peoples R China; [Zhu, Ling; Zeng, Xi; Liu, Weiwei; Han, Wei; Huang, Guoning; Li, Jingyu] Chongqing Hlth Ctr Women & Children, Chongqing Clin Res Ctr Reprod Med, Chongqing, Peoples R China"

通信作者："Huang, GN; Li, JY (通讯作者)，Chongqing Med Univ, Women & Childrens Hosp, Ctr Reprod Med, Chongqing Key Lab Human Embryo Engn, Chongqing, Peoples R China.; Huang, GN; Li, JY (通讯作者)，Chongqing Hlth Ctr Women & Children, Chongqing Clin Res Ctr Reprod Med, Chongqing, Peoples R China."

来源：JOURNAL OF ASSISTED REPRODUCTION AND GENETICS

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000912591200001

JCR分区：Q1

影响因子：3.2

年份：2023

卷号：　

期号：　

开始页：　

结束页：　

文献类型：Article; Early Access

关键词：Single-cell whole-genome bisulfite sequencing; Time-lapse; Standard incubator; Embryo culture

摘要："Purpose We aimed to compare the DNA methylation profiles of human embryos cultured in uninterrupted or interrupted incubators.Methods This study included 9 women, <= 30 years old (range: 20-30 years), without a history of genetic diseases or smoking, undergoing ICSI treatment, and each woman donated one oocyte. Embryos were randomly assigned to culture in either time-lapse imaging or standard incubators after ICSI. We compared the DNA methylation profiles of human eight-cell embryos cultured in uninterrupted condition using time-lapse imaging (TLI) incubator (EmbryoScope) to those cultured in interrupted culture model using standard incubators (SI, G185 K-System). Nine single-cell whole-genome bisulfite sequencing (WGBS) datasets were analyzed, including four SI-cultured embryos and five TLI-cultured embryos at the eight-cell stage.Results A total of 581,140,020 and 732,348,182 clean reads were generated from the TLI and SI groups, respectively. TLI-cultured embryos had similar genome-wide methylation patterns to SI-cultured embryos. There were no significant differences in the methylation and transcription levels of transposable elements and imprinted control regions. Although a total of 198 differentially methylated genes (DMGs) were identified, only five DMGs had significantly different transcription levels between the two groups.Conclusions This is the first study to compare the DNA methylation profiles of embryos cultured in TLI and SI and will provide a foundation for evaluating the safety of TLI application in assisted reproductive technologies. However, further study with a larger cohort of samples was needed for the data validation."

基金机构：Natural Science Foundation of Chongqing [cstc2021jcyj-msxmX0785]

基金资助正文：This work was supported by the Natural Science Foundation of Chongqing (cstc2021jcyj-msxmX0785).