Andrographolide-treated bone marrow mesenchymal stem cells-derived conditioned medium protects cardiomyocytes from injury by metabolic remodeling

作者全名："Sun, Yanting; Xu, Hao; Tan, Bin; Yi, Qin; Liu, Huiwen; Tian, Jie; Zhu, Jing"

作者地址："[Sun, Yanting; Xu, Hao; Tan, Bin; Yi, Qin; Liu, Huiwen; Tian, Jie; Zhu, Jing] Childrens Hosp Chongqing Med Univ, Dept Pediat Res Inst, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, 136 Zhongshan Er Rd, Chongqing 400014, Peoples R China; [Sun, Yanting] First Affiliated Hosp Soochow Univ, Ctr Clin Lab, Suzhou 215006, Peoples R China; [Xu, Hao] Childrens Hosp Chongqing Med Univ, Dept Clin Lab, Chongqing, Peoples R China; [Tian, Jie] Childrens Hosp Chongqing Med Univ, Dept Cardiovasc Internal Med, Chongqing, Peoples R China"

通信作者："Zhu, J (通讯作者)，Childrens Hosp Chongqing Med Univ, Dept Pediat Res Inst, Natl Clin Res Ctr Child Hlth & Disorders, Minist Educ Key Lab Child Dev & Disorders, 136 Zhongshan Er Rd, Chongqing 400014, Peoples R China."

来源：MOLECULAR BIOLOGY REPORTS

ESI学科分类：MOLECULAR BIOLOGY & GENETICS

WOS号：WOS:000912852100001

JCR分区：Q3

影响因子：2.6

年份：2023

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期号：　

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文献类型：Article; Early Access

关键词：Conditioned medium; Cardiomyocytes; Metabolic remodeling; Oxidative stress

摘要："BackgroundBone marrow mesenchymal stem cells (BMSCs) transplantation therapy providing a great hope for the recovery of myocardial ischemic hypoxic injury. However, the microenvironment after myocardial injury is not conducive to the survival of BMSCs, which limits the therapeutic application of BMSCs. Our previous study has confirmed that the survival of BMSCs cells in the glucose and serum deprivation under hypoxia (GSDH) is increased after Andrographolide (AG) pretreatment, but whether this treatment could improve the effect of BMSCs in repairing of myocardial injury has not been verified.Methods and resultWe first treated H9C2 with GSDH to simulate the microenvironment of myocardial injury in vitro, then we pretreated rat primary BMSCs with AG, and collected conditioned medium derived from BMSCs (BMSCs-CM) and conditioned medium derived from AG-pretreated BMSCs (AG-BMSCs-CM) after GSDH treatment. And they were used to treat H9C2 cells under GSDH to further detect oxidative stress and metabolic changes. The results showed that AG-BMSCs-CM could be more advantageous for cardiomyocyte injury repair than BMSCs-CM, as indicated by the decrease of apoptosis rate and oxidative stress. The changes of mitochondria and lipid droplets results suggested that AG-BMSCs-CM can regulate metabolic remodeling of H9C2 cells to repair cell injury, and that AMPK was activated during this process.ConclusionsThis study demonstrates, for the first time, the protective effect of AG-BMSCs-CM on GSDH-induced myocardial cell injury, providing a potential therapeutic strategy for clinical application."

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