"Improvement of antibacterial, anti-inflammatory, and osteogenic properties of OGP loaded Co-MOF coating on titanium implants for advanced osseointegration"

作者全名："Tao, Bailong; Yi, Weiwei; Qin, Xian; Wu, Junjie; Li, Kai; Guo, Ai; Hao, Jie; Chen, Lixue"

作者地址："[Tao, Bailong; Chen, Lixue] Chongqing Med Univ, Lab Res Ctr, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Yi, Weiwei] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 1, Chongqing 400016, Peoples R China; [Qin, Xian] Chongqing Hlth Ctr Women & Children, Dept Obstet & Gynecol, Chongqing 401147, Peoples R China; [Qin, Xian] Chongqing Med Univ, Women & Childrens Hosp, Chongqing 401147, Peoples R China; [Wu, Junjie; Li, Kai; Guo, Ai; Hao, Jie] Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 3, Chongqing 400016, Peoples R China"

通信作者："Tao, BL; Chen, LX (通讯作者)，Chongqing Med Univ, Lab Res Ctr, Affiliated Hosp 1, Chongqing 400016, Peoples R China.; Hao, J (通讯作者)，Chongqing Med Univ, Dept Orthoped, Affiliated Hosp 3, Chongqing 400016, Peoples R China."

来源：JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY

ESI学科分类：MATERIALS SCIENCE

WOS号：WOS:000913001500001

JCR分区：Q1

影响因子：11.2

年份：2023

卷号：146

期号：　

开始页：131

结束页：144

文献类型：Article

关键词：Titanium implants; Osteogenic growth peptide; Anti-inflammatory; Antibacterial activity; Osteoimmunomodulation

摘要："The bacterial infection, especially for methicillin-resistant Staphylococcus aureus (MRSA), and the associated severe inflammatory response could extremely limit the crosstalk of RAW264.7 cells and mesenchymal stem cells (MSCs) and lead to the undesirable osseointegration of peri-implants. It is highly demanded to modify the surface of titanium (Ti) implant to improve its anti-bacterial and anti-inflammatory properties and facilitate its disabled osseointegration. Herein, in our study, a multifunctional coating of zeolitic imidazolate frameworks-67 encapsulated osteogenic growth peptide (OGP) (ZO) was fabricated on titanium dioxide nanotubes (TNT) substrates (TNT-ZO) via the electrophoresis deposition (EPD) approach. The TNT-ZO substrates exhibited excellent antibacterial activity indicated by the reactive oxygen species (ROS) generation, outer membrane (OM) and inner membrane (IM) permeabilization change, adenosine triphosphate (ATP) decrease, and intracellular compounds (DNA/RNA) leakage. Importantly, the regulation effects of TNT-ZO coating modified titanium substrates on the RAW264.7-MSCs crosstalk could induce the anti-inflammatory and osteogenic microenvironment via multiple paracrine signaling of Runx2, BMP2, VEGF, and TGF- beta 1. The promoted effects of coating structure were investigated in vivo, including antibacterial effect, osteogenic differentiation of mesenchymal stem cells, and anti-inflammation of RAW264.7 cells, as well as infected bone regeneration and repair in bone defect transplantation model. The results demonstrated that MRSA was effectively eliminated by the hydrolysis of ZIF-67 nanoparticles on TNT-ZO substrates. Furthermore, the excellent osseointegration of peri-implants was realized simultaneously by modulating the crosstalk of RAW264.7-MSCs. This study could provide a novel approach to designing a multifunctional coating on the Ti implants for infected bone regeneration in orthopedic applications. (c) 2022 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology."

基金机构："National Nat- ural Science Foundation of China [82102537]; Natural Science Foundation of Chongqing Science and Technology Com- mission [cstc2021jcyj-msxmX0170, CSTB2022BSXM-JCX0 039, CSTB2022BSXM-JCX0 058]; First Affiliated Hospital of Chongqing Medical University cultivating fund [PYJJ2021-02, PYJJ2021-04]"

基金资助正文："Acknowledgements This work was financially supported by the National Nat- ural Science Foundation of China (No. 82102537) , the Natural Science Foundation of Chongqing Science and Technology Com- mission (Nos. cstc2021jcyj-msxmX0170, CSTB2022BSXM-JCX0 039, and CSTB2022BSXM-JCX0 058) , the First Affiliated Hospital of Chongqing Medical University cultivating fund (Nos. PYJJ2021-02 and PYJJ2021-04) ."