Aldosterone inhibits Dot1l expression in guinea pig cochlea

作者全名："Zhong, Shixun; Zhang, Biyun; Qin, Li; Wang, Qianying; Luo, Xiaoli"

作者地址："[Zhong, Shixun; Zhang, Biyun; Wang, Qianying] Chongqing Med Univ, Affiliated Hosp 1, Dept Otolaryngol, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Qin, Li] Mianyang Cent Hosp, Dept Otolaryngol, Mianyang, Peoples R China; [Luo, Xiaoli] Chongqing Tradit Chinese Med Hosp, Dept Otolaryngol, Chongqing, Peoples R China"

通信作者："Zhong, SX (通讯作者)，Chongqing Med Univ, Affiliated Hosp 1, Dept Otolaryngol, 1 Youyi Rd, Chongqing 400016, Peoples R China."

来源：EUROPEAN JOURNAL OF MEDICAL RESEARCH

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000913601700001

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：28

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：Dot1l; Aldosterone; Endolymphatic hydrops; Meniere's disease

摘要："BackgroundAldosterone relieves transcriptional repression of epithelial sodium channel (ENaC) by inhibiting Dot1a and Af9 expression and their interaction with ENaC promoter in various tissues. Expressions of ENaC and Af9 in inner ear have been identified. However, it is not known how Dot1l is regulated by aldosterone in inner ear.MethodsTwenty-eight adult guinea pigs were randomly divided into the control group and treatment group. Aldosterone 1 mg/kg/d was injected intraperitoneally in the treatment group and saline in the control group for 7 days. Animals were killed 1 month later following auditory brainstem response examination. Histomorphology of cochlea was detected with hematoxylin-eosin staining, and Dot1l expression was examined with immunohistochemistry and Western blot.ResultsThere was no significant difference in ABR thresholds before and after injection of aldosterone or saline in either group. Endolymphatic hydrops was found in 75% of animals in the treatment group. Dot1l was found in both groups in the stria vascularis, Reissner's membrane, spiral limbus, organ of Corti and spiral ligament. Dot1l expression in the treatment group was decreased by aldosterone.ConclusionsDot1l in guinea pig cochlea is inhibited by aldosterone with induction of endolymphatic hydrops. Dot1l may be closely related to endolymph regulation by aldosterone and to pathogenesis of Meniere's disease."

基金机构："Key Project of Technical Innovation, Application and Development in Health Field of Chongqing Science and Technology Bureau [CSTC2021jscx-gksb-N0012]"

基金资助正文："This work was supported by the Key Project of Technical Innovation, Application and Development in Health Field of Chongqing Science and Technology Bureau (CSTC2021jscx-gksb-N0012)"