"Analysis of the expression and distribution of protein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase 1 in the normal adult mouse brain"

作者全名："Jiang, Hanxiao; Feng, Yuxue; He, Guiqiong; Liu, Yuanjie; Li, Xiaofeng"

作者地址："[Jiang, Hanxiao; Feng, Yuxue; Li, Xiaofeng] Chongqing Med Univ, Dept Neurol, Affiliated Hosp 2, Chongqing, Peoples R China; [He, Guiqiong; Liu, Yuanjie] Chongqing Med Univ, Chongqing Key Lab Neurobiol, Chongqing, Peoples R China; [He, Guiqiong; Liu, Yuanjie] Chongqing Med Univ, Dept Anat, Chongqing, Peoples R China"

通信作者："Li, XF (通讯作者)，Chongqing Med Univ, Dept Neurol, Affiliated Hosp 2, Chongqing, Peoples R China.; Liu, YJ (通讯作者)，Chongqing Med Univ, Chongqing Key Lab Neurobiol, Chongqing, Peoples R China.; Liu, YJ (通讯作者)，Chongqing Med Univ, Dept Anat, Chongqing, Peoples R China."

来源：FRONTIERS IN NEUROANATOMY

ESI学科分类：NEUROSCIENCE & BEHAVIOR

WOS号：WOS:000913784300001

JCR分区：Q1

影响因子：2.1

年份：2023

卷号：16

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：POMGNT1; O-mannosylation; neurons; brain distribution; adult mouse; Golgi

摘要："IntroductionProtein O-linked mannose beta 1,2-N-acetylglucosaminyltransferase 1 (POMGNT1) is crucial for the elongation of O-mannosyl glycans. Mutations in POMGNT1 cause muscle-eye-brain (MEB) disease, one of the main features of which is anatomical aberrations in the brain. A growing number of studies have shown that defects in POMGNT1 affect neuronal migration and distribution, disrupt basement membranes, and misalign Cajal-Retzius cells. Several studies have examined the distribution and expression of POMGNT1 in the fetal or neonatal brain for neurodevelopmental studies in the mouse or human brain. However, little is known about the neuroanatomical distribution and expression of POMGNT1 in the normal adult mouse brain. MethodsWe analyzed the expression of POMGNT1 mRNA and protein in the brains of various neuroanatomical regions and spinal cords by western blotting and RT-qPCR. We also detected the distribution profile of POMGnT1 in normal adult mouse brains by immunohistochemistry and double-immunofluorescence. ResultsIn the present study, we found that POMGNT1-positive cells were widely distributed in various regions of the brain, with high levels of expression in the cerebral cortex and hippocampus. In terms of cell type, POMGNT1 was predominantly expressed in neurons and was mainly enriched in glutamatergic neurons; to a lesser extent, it was expressed in glial cells. At the subcellular level, POMGNT1 was mainly co-localized with the Golgi apparatus, but expression in the endoplasmic reticulum and mitochondria could not be excluded. DiscussionThe present study suggests that POMGNT1, although widely expressed in various brain regions, may has some regional and cellular specificity, and the outcomes of this study provide a new laboratory basis for revealing the possible involvement of POMGNT1 in normal physiological functions of the brain from a morphological perspective."

基金机构：Chongqing Natural Science Fund Key Project [cstc2019jcyj-zdxmX0029]; Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University

基金资助正文：Funding This research was funded by the Chongqing Natural Science Fund Key Project No. cstc2019jcyj-zdxmX0029 and Kuanren Talents Program of the Second Affiliated Hospital of Chongqing Medical University.