A Microfluidic Cell Co-Culture Chip for the Monitoring of Interactions between Macrophages and Fibroblasts

作者全名："Li, Pengcheng; Cui, Feiyun; Chen, Heying; Yang, Yao; Li, Gang; Mao, Hongju; Lyu, Xiaoyan"

作者地址："[Li, Pengcheng] Sichuan Univ, West China Hosp, West China Sch Nursing, Dept Orthoped, Chengdu 610041, Peoples R China; [Cui, Feiyun] Harbin Med Univ, Sch Basic Med Sci, Harbin 150081, Peoples R China; [Chen, Heying] Chongqing Med Univ, Sch Lab Med, Minist Educ, Key Lab Clin Diagnost, Chongqing 400016, Peoples R China; [Yang, Yao; Lyu, Xiaoyan] Sichuan Univ, West China Hosp, Dept Dermatol, Chengdu 610041, Peoples R China; [Yang, Yao; Lyu, Xiaoyan] Sichuan Univ, West China Hosp, Lab Dermatol, Frontiers Sci Ctr Dis,Related Mol Network,Clin Ins, Chengdu 610041, Peoples R China; [Li, Gang] Chongqing Univ, Key Lab Optoelect Technol & Syst, Def Key Disciplines Lab Novel Micronano Devices &, Minist Educ, Chongqing 400044, Peoples R China; [Mao, Hongju] Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China"

通信作者："Lyu, X (通讯作者)，Sichuan Univ, West China Hosp, Dept Dermatol, Chengdu 610041, Peoples R China.; Lyu, X (通讯作者)，Sichuan Univ, West China Hosp, Lab Dermatol, Frontiers Sci Ctr Dis,Related Mol Network,Clin Ins, Chengdu 610041, Peoples R China.; Mao, HJ (通讯作者)，Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, State Key Lab Transducer Technol, Shanghai 200050, Peoples R China."

来源：BIOSENSORS-BASEL

ESI学科分类：　

WOS号：WOS:000914398900001

JCR分区：Q1

影响因子：4.9

年份：2023

卷号：13

期号：1

开始页：　

结束页：　

文献类型：Article

关键词：microfluidic chips; organ-on-a-chip; wound healing; cell co-culture; macrophages

摘要："Macrophages and fibroblasts are two types of important cells in wound healing. The development of novel platforms for studying the interrelationship between these two cells is crucial for the exploration of wound-healing mechanisms and drug development. In this study, a microfluidic chip composed of two layers was designed for the co-culturing of these two cells. An air valve was employed to isolate fibroblasts to simulate the wound-healing microenvironment. The confluence rate of fibroblasts in the co-culture system with different macrophages was explored to reflect the role of different macrophages in wound healing. It was demonstrated that M2-type macrophages could promote the activation and migration of fibroblasts and it can be inferred that they could promote the wound-healing process. The proposed microfluidic co-culture system was designed for non-contact cell-cell interactions, which has potential significance for the study of cell-cell interactions in biological processes such as wound healing, tumor microenvironment, and embryonic development."

基金机构："National Natural Science Foundation of China [81871567, 62231025]"

基金资助正文："This research was funded by the National Natural Science Foundation of China, grant numbers 81871567 and 62231025."