Resveratrol inhibits proliferation and induces apoptosis via the Hippo/ YAP pathway in human colon cancer cells

作者全名："Qin, Xiaoying; Luo, Honghong; Deng, Yixuan; Yao, Xintong; Zhang, Jie; He, Baicheng"

作者地址："[Qin, Xiaoying; Luo, Honghong; Deng, Yixuan; Yao, Xintong; Zhang, Jie; He, Baicheng] Chongqing Med Univ, Yongchuan Hosp, Dept Pharm, Chongqing 402160, Peoples R China; [He, Baicheng] Chongqing Med Univ, Sch Pharm, Dept Pharmacol, Chongqing 400016, Peoples R China; [Qin, Xiaoying; Luo, Honghong; Deng, Yixuan; Yao, Xintong; Zhang, Jie; He, Baicheng] Chongqing Med Univ, Key Lab Biochem & Mol Pharmacol Chongqing, Chongqing 400016, Peoples R China; [He, Baicheng] Chongqing Med Univ, Sch Pharm, Dept Pharmacol, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China"

通信作者："He, BC (通讯作者)，Chongqing Med Univ, Sch Pharm, Dept Pharmacol, 1 Yixueyuan Rd, Chongqing 400016, Peoples R China."

来源：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000914871500006

JCR分区：Q2

影响因子：3.1

年份：2022

卷号：636

期号：　

开始页：197

结束页：204

文献类型：Article

关键词：Resveratrol; Colon cancer; Proliferation inhibition; YAP; Molecular docking

摘要："High malignancy and mortality in colon cancer require clarifying the underlying mechanisms of colon cancer carcinogenesis and exploring new targets or drugs for the clinical treatment of colon cancer. Resveratrol (Res), a natural compound, shows cytotoxicity against various tumors. However, the specific anti-cancer mechanism of Res remains unclear. In the present study, we aimed to explore the anti-cancer activity of Res against colon cancer cells and the possible mechanism. The results showed that Res could inhibit cell proliferation and induce cell cycle arrest and apoptosis in HCT116 cells. Western blotting and Polymerase chain reaction (PCR) showed that Res increased the phosphorylated YAP (pYAP) levels and decreased YAP total protein level and decreased the mRNA expression of the YAP signaling downstream genes CTGF and CYR61. The effects of Res on pYAP were enhanced by YAP inhibitor verteporfin (VP). VP also enhanced the effects of Res on decreasing viability and inducing apoptosis. Furthermore, the molecular docking analysis indicated Res could bind with YAP-TEAD through van der Waals, pi-alkyl, and pipi stacked interactions. Our findings suggested that the anti-cancer activity of Res may be mediated via activating Hippo/YAP signaling and partially disturbing the interaction between YAP and TEAD. All this evidence supports that Res may be an efficacious drug for colon cancer treatment. (c) 2022 Elsevier Inc. All rights reserved."

基金机构：Project of Yongchuan Hospital of Chongqing Medical University; [YJLC202121]

基金资助正文：This work was supported by Project of Yongchuan Hospital of Chongqing Medical University (YJLC202121) .