Controlled released naringin-loaded liposome/sucrose acetate isobutyrate hybrid depot for osteogenesis in vitro and in vivo

作者全名："Meng, Di; Song, Jinlin; Yi, Yin; Li, Jihong; Zhang, Ting; Shu, Yu; Wu, Xiaohong"

作者地址："[Meng, Di; Song, Jinlin; Yi, Yin; Li, Jihong; Zhang, Ting; Shu, Yu; Wu, Xiaohong] Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China; [Meng, Di; Song, Jinlin; Yi, Yin; Li, Jihong; Zhang, Ting; Shu, Yu; Wu, Xiaohong] Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China; [Meng, Di; Song, Jinlin; Yi, Yin; Li, Jihong; Zhang, Ting; Shu, Yu; Wu, Xiaohong] Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China"

通信作者："Wu, XH (通讯作者)，Chongqing Med Univ, Stomatol Hosp, Chongqing, Peoples R China.; Wu, XH (通讯作者)，Chongqing Key Lab Oral Dis & Biomed Sci, Chongqing, Peoples R China.; Wu, XH (通讯作者)，Chongqing Municipal Key Lab Oral Biomed Engn Highe, Chongqing, Peoples R China."

来源：FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY

ESI学科分类：Multidisciplinary

WOS号：WOS:000914930700001

JCR分区：Q1

影响因子：4.3

年份：2023

卷号：10

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：sucrose acetate isobutyrate; anionic liposomes; injectable; local drug delivery; controlled release

摘要："Introduction: A common problem in bone tissue engineering is that the burst release of active osteogenic factors is not beneficial for osteogenesis. This study aimed to prepare naringin (Ng) liposomes to reduce the burst release of Ng and improve new bone formation. Methods: We synthesized Ng liposomes using the thin-film hydration method. Drug-encapsulation efficacy experiments were conducted using the ultracentrifugation technique. The morphology and size distributions of freezedried liposomes were determined by transmission electron microscopy and dynamic light scattering. The Ng liposomes and Ng-lipo/sucrose acetate isobutyrate (SAIB) depots were characterized using Fourier transform infrared spectroscopy and in vitro release studies. After implantation of the Ng-lipo/SAIB depots, in vitro osteoblast-liposome interactions and in vivo osteogenesis were tested. Results: The formulation of freeze-dried Ng liposomes via an optimized recipe yielded nanosized (136.9 nm) negatively charged particles with a high encapsulation efficiency (76.3%). Their chemical structure did not change after adding SAIB to the Ng liposomes. The burst release was reduced dramatically from 74.4% to 23.7%. In vivo, after 8 weeks, the new bone formation rate in the calvarial defects of Sprague-Dawley rats receiving Ng-lipo/SAIB was 57% compared with 25.18% in the control group (p = .0003). Discussion: Our results suggested that Ng-lipo/SAIB hybrid depots could serve as candidate materials for drug delivery in bone regeneration applications."

基金机构：National Natural Science Foundation of China [81970914]

基金资助正文：Funding This work was supported by the National Natural Science Foundation of China (Grant number: 81970914).