Ulinastatin inhibits microglia activation in spinal cord via P2Y12 receptor in a rat neuropathic pain model

作者全名："Shi, Ying; Wen, Huizhong; Cui, Jian; Qin, Wanxiang"

作者地址："[Shi, Ying] Chongqing Med Univ, Affiliated Hosp 1, Dept Pain Care, Chongqing, Peoples R China; [Wen, Huizhong] Army Med Univ, Dept Neurobiol, Chongqing, Peoples R China; [Cui, Jian; Qin, Wanxiang] Army Med Univ, Southwest Hosp, Dept Pain Care, Chongqing, Peoples R China; [Cui, Jian; Qin, Wanxiang] Army Med Univ, Southwest Hosp, Dept Pain Care, 30 Gaotanyan Rd, Chongqing, Peoples R China"

通信作者："Cui, J; Qin, WX (通讯作者)，Army Med Univ, Southwest Hosp, Dept Pain Care, 30 Gaotanyan Rd, Chongqing, Peoples R China."

来源：JOURNAL OF HISTOTECHNOLOGY

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000915290800001

JCR分区：Q4

影响因子：0.6

年份：2023

卷号：46

期号：1

开始页：39

结束页：53

文献类型：Article

关键词：Ulinastatin; neuropathic pain; NF-kappa B

摘要："Ulinastatin, a broad spectrum of serine protease inhibitor, has been found to alleviate neuropathic pain (NPP). However, its mechanism is not completely clear. Here, a sciatic nerve ligation rat model and BV2 microglial cells were used to investigate the effect of Ulinastatin on the activation of microglia and P2Y12 receptors in vivo and in vitro. Levels of P2Y12 receptor and NF-kappa B (P65) expression in the dorsal horn of the lumbar enlargement region of the spinal cord and BV2 cells were assessed by immunohistochemistry and double-label immunofluorescence assays. Levels of IL-1 beta and TNF-alpha in cell culture medium and cerebrospinal fluid (CSF) were examined by ELISA. The results showed that Ulinastatin reduced the release of inflammatory IL-1 beta and TNF-alpha by inhibiting the activation of spinal microglia. Ulinastatin down-regulated P2Y12 receptor and NF-kappa B (P65) expression in the spinal microglia of the chronic constrictive injury model. The results indicated that Ulinastatin may attenuate the activation of spinal microglia after peripheral nerve injury by inhibiting the activation of P2Y12 receptor signal pathway in microglia. NF-kB may play a key role in the mechanism of Ulinastatin."

基金机构：Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0542]; Chongqing Science and health joint medical research project [2021MSXM130]

基金资助正文：This work is sponsored by Natural Science Foundation of Chongqing [cstc2019jcyj-msxmX0542] and Chongqing Science and health joint medical research project [2021MSXM130].