"Single-cell RNA sequencing reveals the molecular features of peripheral blood immune cells in children, adults and centenarians"

作者全名："Zhong, Jinjie; Ding, Rong; Jiang, Huimin; Li, LongFei; Wan, Junli; Feng, Xiaoqian; Chen, Miaomiao; Peng, Liping; Li, Xiaoqin; Lin, Jing; Yang, Haiping; Wang, Mo; Li, Qiu; Chen, Qilin"

作者地址："[Zhong, Jinjie; Jiang, Huimin; Wan, Junli; Feng, Xiaoqian; Chen, Miaomiao; Peng, Liping; Li, Xiaoqin; Lin, Jing; Yang, Haiping; Wang, Mo; Li, Qiu; Chen, Qilin] Chongqing Med Univ, Dept Nephrol, Childrens Hosp, Chongqing, Peoples R China; [Zhong, Jinjie; Jiang, Huimin; Wan, Junli; Feng, Xiaoqian; Chen, Miaomiao; Peng, Liping; Li, Xiaoqin; Lin, Jing; Yang, Haiping; Wang, Mo; Li, Qiu; Chen, Qilin] Minist Educ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Chongqing, Peoples R China; [Zhong, Jinjie; Jiang, Huimin; Feng, Xiaoqian; Peng, Liping; Yang, Haiping; Wang, Mo; Li, Qiu; Chen, Qilin] Chongqing Key Lab Pediat, Chongqing, Peoples R China; [Ding, Rong; Li, LongFei] Nanjing Jiangbei New Area Biopharmaceut Publ Serv, Nanjing, Jiangsu, Peoples R China"

通信作者："Li, Q; Chen, QL (通讯作者)，Chongqing Med Univ, Dept Nephrol, Childrens Hosp, Chongqing, Peoples R China.; Li, Q; Chen, QL (通讯作者)，Minist Educ, Natl Clin Res Ctr Child Hlth & Disorders, Key Lab Child Dev & Disorders, Chongqing, Peoples R China.; Li, Q; Chen, QL (通讯作者)，Chongqing Key Lab Pediat, Chongqing, Peoples R China."

来源：FRONTIERS IN IMMUNOLOGY

ESI学科分类：IMMUNOLOGY

WOS号：WOS:000917690600001

JCR分区：Q1

影响因子：5.7

年份：2023

卷号：13

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：single-cell RNA sequencing (scRNAseq); peripheral blood mononuclear cells; whole lifespan; autoimmune diseases; CD8(+) cytotoxic T cells

摘要："Peripheral blood immune cells have different molecular characteristics at different stages of the whole lifespan. Knowledge of circulating immune cell types and states from children to centenarians remains incomplete. We profiled peripheral blood mononuclear cells (PBMCs) of multiple age groups with single-cell RNA sequencing (scRNA-seq), involving the age ranges of 1-12 (G1), 20-30(G2), 30-60(G3), 60-80(G4), and >110 years (G5). The proportion and states of myeloid cells change significantly from G1 to G2. We identified a novel CD8(+)CCR7(+)GZMB(+) cytotoxic T cell subtype specific in G1, expressing naive and cytotoxic genes, and validated by flow cytometry. CD8(+) T cells showed significant changes in the early stage (G1 to G2), while CD4(+) T cells changed in the late stage (G4 to G5). Moreover, the intercellular crosstalk among PBMCs in G1 is very dynamic. Susceptibility genes for a variety of autoimmune diseases (AIDs) have different cell-specific expression localization, and the expression of susceptibility genes for AIDs changes with age. Notably, the CD3(+) undefined T cells clearly expressed susceptibility genes for multiple AIDs, especially in G3. ETS1 and FLI1, susceptibility genes associated with systemic lupus erythematosus, were differentially expressed in CD4(+) and CD8(+) effector cells in G1 and G3. These results provided a valuable basis for future research on the unique immune system of the whole lifespan and AIDs."

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