Cell Cycle-Related Gene SPC24: A Novel Potential Diagnostic and Prognostic Biomarker for Laryngeal Squamous Cell Cancer

作者全名："Chen, Jialei; Luo, Jing; He, Jing; Jiang, Xianyao; Jiang, Ning; Yang, Changhong; Zhong, Shixun"

作者地址："[Chen, Jialei; He, Jing; Jiang, Xianyao; Zhong, Shixun] Chongqing Med Univ, Dept Otolaryngol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China; [Chen, Jialei] Chongqing Med Univ, Dept Pathol & Pathophysiol, Chongqing, Peoples R China; [Chen, Jialei; Luo, Jing] Chongqing Med Univ, Coll Basic Med Sci, Mol Med & Canc Res Ctr, Chongqing 400016, Peoples R China; [Luo, Jing; Jiang, Ning] Chongqing Med Univ, Dept Pathol, Chongqing, Peoples R China; [Luo, Jing; Jiang, Ning] Chongqing Med Univ, Dept Pathol, Affiliated Hosp 1, Chongqing, Peoples R China; [Yang, Changhong] Chongqing Med Univ, Coll Basic Med, Dept Bioinformat, Chongqing 400016, Peoples R China"

通信作者："Zhong, SX (通讯作者)，Chongqing Med Univ, Dept Otolaryngol, Affiliated Hosp 1, 1 Youyi Rd, Chongqing 400016, Peoples R China.; Yang, CH (通讯作者)，Chongqing Med Univ, Coll Basic Med, Dept Bioinformat, Chongqing 400016, Peoples R China."

来源：BIOMED RESEARCH INTERNATIONAL

ESI学科分类：BIOLOGY & BIOCHEMISTRY

WOS号：WOS:000919203100001

JCR分区：Q3

影响因子：2.6

年份：2023

卷号：2023

期号：　

开始页：　

结束页：　

文献类型：Article

关键词：　

摘要："Laryngeal squamous cell cancer (LSCC) is a common malignant tumor with a high degree of malignancy, and its etiology remains unclear. Therefore, screening potential biomarkers is necessary to facilitate the treatment and diagnosis of LSCC. Robust rank aggregation (RRA) analysis was used to integrate two gene expression datasets of LSCC patients from the Gene Expression Omnibus (GEO) database and identify differentially expressed genes (DEGs) between LSCC and nonneoplastic tissues. A gene coexpression network was constructed using weighted gene correlation network analysis (WGCNA) to explore potential associations between the module genes and clinical features of LSCC. Combining differential gene expression analysis and survival analysis, we screened potential hub genes, including CDK1, SPC24, HOXB7, and SELENBP1. Subsequently, western blotting and immunohistochemistry were used to test the protein levels in clinical specimens to verify our findings. Finally, four candidate diagnostic and prognostic biomarkers (CDK1, SPC24, HOXB7, and SELENBP1) were identified. We propose, for the first time, that SPC24 is a gene that may associate with LSCC malignancy and is a novel therapeutic target. These findings may provide important mechanistic insight of LSCC."

基金机构："National Natural Science Foundation of Chongqing of China [cstc2020jcyj-bshX0069]; Natural Science Foundation of Chongqing of China [cstc2019jcyj-msxm0842]; Key Project of Technical Innovation, Application and Development in Health Field of Chongqing [CSTC2021jscx-gksb-N0012]"

基金资助正文："AcknowledgmentsThis work was supported by the National Natural Science Foundation of Chongqing of China (No. cstc2020jcyj-bshX0069) and the Natural Science Foundation of Chongqing of China (cstc2019jcyj-msxm0842), and the Key Project of Technical Innovation, Application and Development in Health Field of Chongqing (CSTC2021jscx-gksb-N0012)."