B7-H6 enhances F-actin rearrangement by targeting c-MYC activation to promote medulloblastoma migration and invasion

作者全名："Fan, Jianing; Wang, Yangyang; Liang, Xiao; Peng, Yan; Li, Shijie; Li, Xiaoju; Zhou, Fanlin; Li, Yu"

作者地址："[Fan, Jianing; Liang, Xiao; Li, Yu] Chongqing Univ, Sch Med, Chongqing, Peoples R China; [Wang, Yangyang] Bioengn Coll Chongqing Univ, Chongqing, Peoples R China; [Peng, Yan] Chongqing Med Univ, Inst Neurosci, Sch Basic Med, Chongqing, Peoples R China; [Fan, Jianing; Wang, Yangyang; Liang, Xiao; Li, Shijie; Li, Xiaoju; Zhou, Fanlin; Li, Yu] Chongqing Univ Canc Hosp, Dept Pathol, Chongqing, Peoples R China; [Fan, Jianing; Wang, Yangyang; Liang, Xiao; Li, Shijie; Li, Xiaoju; Zhou, Fanlin; Li, Yu] Chongqing Canc Inst, Chongqing, Peoples R China; [Fan, Jianing; Wang, Yangyang; Liang, Xiao; Li, Shijie; Li, Xiaoju; Zhou, Fanlin; Li, Yu] Chongqing Canc Hosp, Chongqing, Peoples R China"

通信作者："Li, Y (通讯作者)，Chongqing Univ, Sch Med, Chongqing, Peoples R China.; Li, Y (通讯作者)，Chongqing Univ Canc Hosp, Dept Pathol, Chongqing, Peoples R China.; Li, Y (通讯作者)，Chongqing Canc Inst, Chongqing, Peoples R China.; Li, Y (通讯作者)，Chongqing Canc Hosp, Chongqing, Peoples R China."

来源：MEDICAL ONCOLOGY

ESI学科分类：CLINICAL MEDICINE

WOS号：WOS:000920059100001

JCR分区：Q2

影响因子：2.8

年份：2023

卷号：40

期号：3

开始页：　

结束页：　

文献类型：Article

关键词：B7-H6; c-MYC; F-actin; Medulloblastoma; Migration and invasion

摘要："Medulloblastoma (MB) is children's most common primary malignant primitive neuro-ectodermal tumor. Group 3 MB showed a higher propensity to metastasis, which is molecularly characterized by c-MYC gene amplification. The activation of c-MYC promotes the remodeling of the F-actin cytoskeleton to enhance metastasis. The B7 homologue 6 (B7-H6) is associated with the manifold essential hallmarks of tumorigenesis. In this study, we will explore whether B7-H6 regulates the reorganization of F-actin by elevating the c-MYC expression to promote metastasis. The Daoy cell line was used to act as the cell model of medulloblastoma. Small interfering RNA and the plasmid were used to downregulate and upregulate the expression of B7-H6 in Daoy cells. Transwell assays with/without the matrigel matrix were used to detect migration and invasion of Daoy cells. Western blots were used to detect the expression of related proteins. Immunofluorescence staining was used to observe the impact of B7-H6 on the c-MYC /F-actin axis. B7-H6 improved migration and invasion in the Daoy cell line. B7-H6 enhanced the rearrangement of F-actin and activated the expression of MMP-9 and MMP-2. B7-H6 promoted the remodeling of F-actin by targeting c-MYC activation to reinforce migration and invasion. B7-H6 acts as a promoter of migration and invasion in medulloblastoma by activating the c-MYC /F-actin axis."

基金机构："Performance Incentive Guidance for Scientific Research Institution of Chongqing [cstc2020jxjl30013]; Graduate Research and Innovation Foundation of Chongqing, China [CYS22138]; Fundamental Research Funds for the Central University [2019CDYGZD002]"

基金资助正文："This study was supported by the Performance Incentive Guidance for Scientific Research Institution of Chongqing (No. cstc2020jxjl30013), the Graduate Research and Innovation Foundation of Chongqing, China (No. CYS22138), and Fundamental Research Funds for the Central University (No. 2019CDYGZD002)."